Steglich Claudia, Stazic Damir, Lott Steffen C, Voigt Karsten, Greengrass Eyal, Lindell Debbie, Hess Wolfgang R
Genetics and Experimental Bioinformatics Group, Faculty of Biology, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany.
Genetics and Experimental Bioinformatics Group, Faculty of Biology, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany.
Mar Genomics. 2015 Feb;19:5-7. doi: 10.1016/j.margen.2014.10.009. Epub 2014 Nov 4.
Regulatory RNAs play a central role in the regulation of gene expression and can act on several regulatory levels from transcriptional initiation and RNA processing to the control of initiation of translation and RNA stability. One class of these molecules is non-coding (nc)RNAs in bacteria that typically lack protein-coding potential, range in size between 50 and 500nt and originate from intergenic regions. Common methods for the identification of these RNAs are either based on computational predictions, or on transcriptomic analyses of laboratory cultures, whereas very little is known about ncRNAs in environmental microbial populations. Here, we have combined a metatranscriptomics approach with a selective enrichment protocol for ncRNAs. The primary objective of this study was the identification of novel, environmentally relevant ncRNAs focusing on the cyanobacterium Prochlorococcus, which was one of the dominant microorganisms of the marine community of the Gulf of Aqaba when samples were taken.
调控RNA在基因表达调控中发挥着核心作用，并且可以在从转录起始、RNA加工到翻译起始控制和RNA稳定性控制的多个调控层面发挥作用。这类分子中的一类是细菌中的非编码（nc）RNA，其通常缺乏蛋白质编码潜力，大小在50至500个核苷酸之间，起源于基因间区域。鉴定这些RNA的常用方法要么基于计算预测，要么基于实验室培养物的转录组分析，而对于环境微生物群体中的ncRNA知之甚少。在这里，我们将宏转录组学方法与ncRNA的选择性富集方案相结合。本研究的主要目的是鉴定新型的、与环境相关的ncRNA，重点是原绿球藻，在采集样本时，它是亚喀巴湾海洋群落的主要微生物之一。
