Marti Gerardo Aníbal, Balsalobre Agustín, Susevich María Laura, Rabinovich Jorge Eduardo, Echeverría María Gabriela
Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET-UNLP), Boulevard 120 e/61 y 62, 1900 La Plata, Argentina; Researchers of CONICET (CCT-La Plata), 8#1467, 1900 La Plata, Argentina.
Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET-UNLP), Boulevard 120 e/61 y 62, 1900 La Plata, Argentina; Fellow of CONICET (CCT-La Plata), 8#1467, 1900 La Plata, Argentina.
J Invertebr Pathol. 2015 Jan;124:57-60. doi: 10.1016/j.jip.2014.10.008. Epub 2014 Nov 4.
Triatoma virus (TrV) is the only triatomine entomopathogenic virus identified so far. Propagation of TrV in insectaries depends on handling procedures and triatomine population dynamics. The effects of propagation can be devastating and entire colonies must often be sacrificed to prevent spread of the virus throughout the insectary. This study found that after 41.3 days from TrV ingestion of human blood with 0.04 mg of viral protein by 5th instar Triatomainfestans, viral particles could be detected by RT-PCR; in a second horizontal transmission experiment time to detection resulted in a mean of 42.5 days. These results should rise awareness of TrV dynamics in nature, help estimate the spread of this virus when TrV-infected field-collected insects are incorporated into an insectary, and provide a base for the consideration of TrV as an agent of biological control of some species of triatomines.
锥蝽病毒（TrV）是目前已鉴定出的唯一一种锥蝽昆虫病原病毒。TrV在昆虫饲养室中的传播取决于处理程序和锥蝽种群动态。传播的影响可能是毁灭性的，为防止病毒在整个昆虫饲养室传播，常常必须牺牲整个群体。本研究发现，五龄骚扰锥蝽摄入含0.04毫克病毒蛋白的人血41.3天后，可通过逆转录聚合酶链反应（RT-PCR）检测到病毒颗粒；在第二项水平传播实验中，检测到病毒颗粒的平均时间为42.5天。这些结果应提高人们对TrV在自然界中动态的认识，有助于估计当将野外采集的感染TrV的昆虫引入昆虫饲养室时该病毒的传播情况，并为将TrV视为某些锥蝽物种生物防治因子的考虑提供依据。
