Honda S, Suzuki K, Kakehi K
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-osaka, Japan.
Anal Biochem. 1989 Feb 15;177(1):62-6. doi: 10.1016/0003-2697(89)90014-6.
Iodate and periodate were rapidly (in 11 min) separated from each other with high column efficiency by capillary zone electrophoresis, using a fused silica tube (50 microns i.d., 80 cm) and 100 mM acetate buffer, pH 4.5, as carrier. On-column uv detection at 222 nm allowed sensitive detection down to the picomole level, and measurement of relative peak area to that of pyromellitic acid (internal standard) enabled reproducible determination of these ions. This method was proved useful for periodate oxidation analysis of various carbohydrates.
