Urata Yoshishige, Goto Shinji, Luo Lan, Doi Hanako, Kitajima Yuriko, Masuda Shinya, Ono Yusuke, Li Tao-Sheng
Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Biochem Biophys Res Commun. 2014 Nov 21;454(3):376-80. doi: 10.1016/j.bbrc.2014.10.090. Epub 2014 Oct 24.
Although stem cells are generally thought to be resistant to oxidative stress, the fact and in detail molecular mechanism are still to be clearly identified. We herein tried to understand the overall characterization of redox regulatory signaling in hematopoietic stem cells. We purified c-kit-positive hematopoietic stem/progenitor cells from the bone marrow of healthy mice, and then evaluated their redox regulatory property. Compared to the c-kit-negative matured mononuclear cells, c-kit-positive stem/progenitor cells showed lower basic levels of intracellular reactive oxygen species, faster clearance of the accumulated intracellular reactive oxygen species, and higher resistant to oxidative stress. An overall view on the gene expression profile associated with redox regulation showed to be widely differed between cell types. We confirmed that the c-kit-positive stem/progenitor cells expressed significantly higher of Nox1 and catalase, but less of lactoperoxidase than these matured mononuclear cells. Our data suggests that stem cells keep specific redox regulatory property for defensing against oxidative stress.
尽管干细胞通常被认为对氧化应激具有抗性，但这一事实及其详细的分子机制仍有待明确鉴定。我们在此试图了解造血干细胞中氧化还原调节信号的总体特征。我们从健康小鼠的骨髓中纯化出c-kit阳性造血干/祖细胞，然后评估它们的氧化还原调节特性。与c-kit阴性成熟单核细胞相比，c-kit阳性干/祖细胞表现出较低的细胞内活性氧基础水平、更快清除积累的细胞内活性氧以及更高的氧化应激抗性。对与氧化还原调节相关的基因表达谱的总体观察表明，不同细胞类型之间存在广泛差异。我们证实c-kit阳性干/祖细胞表达的Nox1和过氧化氢酶明显高于这些成熟单核细胞，但乳过氧化物酶的表达则较少。我们的数据表明，干细胞具有特定的氧化还原调节特性以抵御氧化应激。
