Pereira P A, Vilela M, Sousa S, Neves J, Paula-Barbosa M M, Madeira M D
Department of Anatomy, Faculty of Medicine, University of Porto, Alameda Professor Hernâni Monteiro, 4200-319 Porto, Portugal; Center of Experimental Morphology (CME), Faculty of Medicine, University of Porto, Alameda Professor Hernâni Monteiro, 4200-319 Porto, Portugal; Center for Health Technology and Services Research (CINTESIS), Faculty of Medicine, University of Porto, Rua Dr. Plácido da Costa, 4200-450 Porto, Portugal.
Department of Anatomy, Faculty of Medicine, University of Porto, Alameda Professor Hernâni Monteiro, 4200-319 Porto, Portugal; Center of Experimental Morphology (CME), Faculty of Medicine, University of Porto, Alameda Professor Hernâni Monteiro, 4200-319 Porto, Portugal.
Neuroscience. 2015 Jan 22;284:707-718. doi: 10.1016/j.neuroscience.2014.10.054. Epub 2014 Nov 5.
The effects of the ibotenic acid infused into the area of the laterodorsal tegmental nucleus (LDT) of rats on the expression of cortical and accumbal neuropeptides were assessed. The effects of this manipulation were determined in the nucleus accumbens (NAc) and medial prefrontal cortex (mPFC) by estimating the numerical density of varicosities immunoreactive for vesicular acetylcholine transporter and the total number of NAc neurons immunoreactive for choline acetyltransferase (ChAT) and neuropeptide Y (NPY) as well as the total number of mPFC neurons immunoreactive for NPY and vasoactive intestinal polypeptide (VIP). In LDT-lesioned rats, the density of the cholinergic varicosities was reduced in the ventral divisions of the mPFC and in all divisions of the NAc. In addition, in these rats, the total number of NPY-immunoreactive neurons was reduced in all subregions of the mPFC and in the NAc. Conversely, the total number of VIP-immunoreactive neurons in the mPFC and of ChAT-immunoreactive neurons in the NAc did not differ between LDT- and sham-lesioned rats. These data provide the first direct evidence for a relationship between selective damage of LDT cholinergic neurons and decreased expression of NPY in the mPFC and NAc. They also reveal that different types of cortical and accumbal interneurons respond differently to the cholinergic denervation induced by LDT lesions.
评估了向大鼠背外侧被盖核（LDT）区域注入鹅膏蕈氨酸对皮质和伏隔核神经肽表达的影响。通过估计对囊泡乙酰胆碱转运体免疫反应性的膨体的数值密度以及对胆碱乙酰转移酶（ChAT）和神经肽Y（NPY）免疫反应性的伏隔核（NAc）神经元总数，以及对NPY和血管活性肠多肽（VIP）免疫反应性的内侧前额叶皮质（mPFC）神经元总数，来确定这种操作在伏隔核（NAc）和内侧前额叶皮质（mPFC）中的效果。在LDT损伤的大鼠中，mPFC腹侧区和NAc所有分区中胆碱能膨体的密度降低。此外，在这些大鼠中，mPFC所有亚区和NAc中NPY免疫反应性神经元的总数减少。相反，LDT损伤和假损伤大鼠之间，mPFC中VIP免疫反应性神经元总数和NAc中ChAT免疫反应性神经元总数没有差异。这些数据为LDT胆碱能神经元的选择性损伤与mPFC和NAc中NPY表达降低之间的关系提供了首个直接证据。它们还揭示了不同类型的皮质和伏隔核中间神经元对LDT损伤诱导的胆碱能去神经支配反应不同。
