López-García Kenia, Cuevas Estela, Sánchez-García Octavio, Pacheco Pablo, Martínez-Gómez Margarita, Castelán Francisco
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
Doctorado en Neuroetología, Universidad Veracruzana, Xalapa, México.
Neurourol Urodyn. 2016 Feb;35(2):180-5. doi: 10.1002/nau.22702. Epub 2014 Nov 28.
To determine the extent of damage and regeneration associated with multiparity on the pubococcygeus and bulbospongiosus muscles.
Age-matched virgin nulliparous and multiparous rabbits that were killed at days 3 and 20 after the fourth delivery were used to harvest pubococcygeus and bulbospongiosus muscles. The activity of the lysosomal enzyme β-glucuronidase was used as a muscle damage indicator. The number of immunoreactive myofiber-associated nuclei anti-Pax7, -MyoD, and -myogenin, as well as the anti-desmin immunoreactive area were measured in muscle sections to estimate some regenerative stages. Significant differences were considered at a P ≤ 0.05.
The β-glucuronidase activity was increased at postpartum day 20 in the pubococcygeus muscle. This variable was unaltered in the bulbospongiosus muscles of multiparas regardless of the postpartum day on which this was measured. The number of immunoreactive nuclei anti-Pax7 in the pubococcygeus muscle was similar between nulliparas and multiparas, whilst those of anti-MyoD and anti-myogenin were increased at postpartum days 3 and 20. The same was true for these latter three markers evaluated in the bulbospongiosus muscles, supporting an ongoing regeneration. The desmin-positive percentage of muscle area per field was increased at postpartum day 20 in the pubococcygeus muscle, whilst such an increment was seen at postpartum days 3 and 20 in the bulbospongiosus muscles.
Damage and regeneration of the pubococcygeus and bulbospongiosus muscles are differently influenced by multiparity in rabbits. This could rely on the anatomical location, metabolism, myofiber composition, and muscle exertion during pregnancy and/or the delivery of each muscle.
确定经产对耻骨尾骨肌和球海绵体肌造成的损伤程度以及与之相关的再生情况。
选取年龄匹配的未生育处女兔和经产兔，在第四次分娩后第3天和第20天处死，用于获取耻骨尾骨肌和球海绵体肌。溶酶体酶β-葡萄糖醛酸酶的活性用作肌肉损伤指标。在肌肉切片中测量抗Pax7、抗MyoD和抗生肌蛋白的免疫反应性肌纤维相关核的数量，以及抗结蛋白免疫反应性面积，以评估一些再生阶段。P≤0.05时认为存在显著差异。
产后第20天，耻骨尾骨肌中的β-葡萄糖醛酸酶活性增加。无论在产后第几天测量，经产兔球海绵体肌中的该变量均未改变。未生育兔和经产兔耻骨尾骨肌中抗Pax7免疫反应性核的数量相似，而抗MyoD和抗生肌蛋白的数量在产后第3天和第20天增加。在球海绵体肌中评估的后三种标志物也是如此，支持正在进行的再生。耻骨尾骨肌中每视野肌肉面积的结蛋白阳性百分比在产后第20天增加，而球海绵体肌在产后第3天和第20天出现这种增加。
经产对兔耻骨尾骨肌和球海绵体肌的损伤和再生有不同影响。这可能取决于每块肌肉在妊娠和/或分娩期间的解剖位置、代谢、肌纤维组成和肌肉用力情况。
