碳氮源进料策略对裂殖壶菌 LU310 高产生物质和二十二碳六烯酸（DHA）的影响。
Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp. LU310.
机构信息
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China; Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
出版信息
Bioresour Technol. 2015 May;184:139-147. doi: 10.1016/j.biortech.2014.09.130. Epub 2014 Oct 7.
A new isolated Schizochytrium sp. LU310 from the mangrove forest of Wenzhou, China, was found as a high producing microalga of docosahexaenoic acid (DHA). In this study, the significant improvements for DHA fermentation by the batch mode in the baffled flasks (i.e. higher oxygen supply) were achieved. By applied the nitrogen-feeding strategy in 1000 mL baffled flasks, the biomass, DHA concentration and DHA productivity were increased by 110.4%, 117.9% and 110.4%, respectively. Moreover, DHA concentration of 21.06 g/L was obtained by feeding 15 g/L of glucose intermittently, which was an increase of 41.25% over that of the batch mode. Finally, an innovative strategy was carried out by intermittent feeding carbon and simultaneously feeding nitrogen. The maximum DHA concentration and DHA productivity in the fed-batch cultivation reached to 24.74 g/L and 241.5 mg/L/h, respectively.
从中国温州红树林中发现了一种新的隔裂壶菌 LU310，它是二十二碳六烯酸（DHA）的高产微藻。在这项研究中，通过在有挡板的摇瓶中分批培养（即提供更高的氧气供应）实现了 DHA 发酵的显著提高。通过在 1000 mL 有挡板的摇瓶中应用氮源补料策略，生物量、DHA 浓度和 DHA 生产力分别提高了 110.4%、117.9%和 110.4%。此外，通过间歇添加 15 g/L 的葡萄糖，DHA 浓度达到 21.06 g/L，比分批培养提高了 41.25%。最后，通过间歇补料碳和同时补料氮实施了一种创新策略。在补料分批培养中，DHA 浓度和 DHA 生产力的最大值分别达到 24.74 g/L 和 241.5 mg/L/h。
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