Degtiarev S Kh, Belavin P A, Shishkina I G, Zarytova V F, Gavriuchenkova L P
Bioorg Khim. 1989 Mar;15(3):358-62.
Affinity chromatography of IIS type restriction endonucleases is proposed. It is shown that endonucleases HgaI, FokI, and SfaNI have affinity to the matrix with immobilized oligonucleotides which contain the endonuclease's recognition sites resistant to the hydrolysis.
提出了IIS型限制性内切核酸酶的亲和色谱法。结果表明,内切核酸酶HgaI、FokI和SfaNI对含有对水解具有抗性的内切核酸酶识别位点的固定化寡核苷酸的基质具有亲和力。