低强度激光疗法通过抑制多形核中性粒细胞黏附来减轻脂多糖诱导的大鼠乳腺炎。
Low-level laser therapy attenuates LPS-induced rats mastitis by inhibiting polymorphonuclear neutrophil adhesion.
作者信息
Wang Yueqiang, He Xianjing, Hao Dandan, Yu Debin, Liang Jianbin, Qu Yanpeng, Sun Dongbo, Yang Bin, Yang Keli, Wu Rui, Wang Jianfa
机构信息
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, P.R. China.
出版信息
J Vet Med Sci. 2014 Nov;76(11):1443-50. doi: 10.1292/jvms.14-0061. Epub 2014 Aug 22.
The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on a rat model of lipopolysaccharide (LPS)-induced mastitis and its underlying molecular mechanisms. The rat model of mastitis was induced by inoculation of LPS through the canals of the mammary gland. The results showed that LPS-induced secretion of IL-1β and IL-8 significantly decreased after LLLT (650 nm, 2.5 mW, 30 mW/cm(2)). LLLT also inhibited intercellular adhesion molecule-1 (ICAM-1) expression and attenuated the LPS-induced decrease of the expression of CD62L and increase of the expression of CD11b. Moreover, LLLT also suppressed LPS-induced polymorphonuclear neutrophils (PMNs) entering the alveoli of the mammary gland. The number of PMNs in the mammary alveolus and the myeloperoxidase (MPO) activity were decreased after LLLT. These results suggested that LLLT therapy is beneficial in decreasing the somatic cell count and improving milk nutritional quality in cows with an intramammary infection.
本研究旨在探讨低强度激光疗法（LLLT）对脂多糖（LPS）诱导的大鼠乳腺炎模型的影响及其潜在分子机制。通过乳腺导管接种LPS诱导大鼠乳腺炎模型。结果显示，低强度激光疗法（650nm，2.5mW，30mW/cm²）后，LPS诱导的IL-1β和IL-8分泌显著减少。低强度激光疗法还抑制细胞间黏附分子-1（ICAM-1）表达，并减轻LPS诱导的CD62L表达降低和CD11b表达增加。此外，低强度激光疗法还抑制LPS诱导的多形核中性粒细胞（PMN）进入乳腺肺泡。低强度激光疗法后，乳腺肺泡中的PMN数量和髓过氧化物酶（MPO）活性降低。这些结果表明，低强度激光疗法有助于降低患有乳房内感染奶牛的体细胞计数并改善牛奶营养质量。
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