Meunier B, Colson A M
Université Catholique de Louvain, Laboratoire de Génétique Microbienne, Belgium.
Curr Genet. 1989 Feb;15(2):121-7. doi: 10.1007/BF00435458.
In Saccharomyces cerevisiae, diuron blocks the respiratory pathway at the level of the bc1 complex. Two mitochondrially inherited loci, DIU1 and DIU2, located in the cytochrome b gene, and two nuclearly inherited loci, DIU3 and DIU4, have previously been identified. The present work genetically characterizes two double mutants. One mutant, Diu-217, carries two nuclearly inherited mutations, diu3-217a and diu-217b; the second mutant, Diu-783, carries the previously described nuclear mutation diu3-783 and a mitochondrial mutation diu2-783. Each mutation, independent of its location, exhibits a weak diuron resistance. The joint expression of two or three mutations leads to a cumulative or a cooperative enhanced diuron-resistant phenotype.
在酿酒酵母中，敌草隆在bc1复合体水平阻断呼吸途径。先前已鉴定出位于细胞色素b基因中的两个线粒体遗传位点DIU1和DIU2，以及两个核遗传位点DIU3和DIU4。目前的工作对两个双突变体进行了遗传学特征分析。一个突变体Diu - 217携带两个核遗传突变，diu3 - 217a和diu - 217b；第二个突变体Diu - 783携带先前描述的核突变diu3 - 783和线粒体突变diu2 - 783。每个突变，无论其位置如何，都表现出较弱的敌草隆抗性。两个或三个突变的联合表达导致累积或协同增强的敌草隆抗性表型。
