通过氧化还原电势转移控制策略，对詹氏丙酸杆菌进行代谢工程改造以提高丙酸产量。
Improved propionic acid production with metabolically engineered Propionibacterium jensenii by an oxidoreduction potential-shift control strategy.
机构信息
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Wuxi 214122, China.
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta 30332, USA.
出版信息
Bioresour Technol. 2015 Jan;175:606-12. doi: 10.1016/j.biortech.2014.10.038. Epub 2014 Oct 22.
In this study, a three-stage oxidoreduction potential (ORP) control strategy was developed to improve propionic acid (PA) production using engineered Propionibacterium jensenii ATCC 4868 (pZGX04-gldA) in a 3-L bioreactor. Specifically, ORP was controlled at -200mV from 0 to 36h, -300mV from 36 to 156h, and -400mV after 156h. The PA titer increased from 21.38 to 27.31g/L. The effects of ORP regulation on key intracellular metabolites were studied, demonstrating that ORP can both regulate NADH/NAD(+) ratio and the activities of some enzymes involved in electron transport and redistribute metabolic flux. We integrated the ORP control strategy with a fed-batch culture method and increased PA production to 39.53g/L. This new ORP control strategy may be useful in the optimization of other anaerobic processes.
在这项研究中，开发了一种三阶段氧化还原电位 (ORP) 控制策略，以提高工程丙酸棒状杆菌 ATCC 4868（pZGX04-gldA）在 3-L 生物反应器中生产丙酸 (PA) 的产量。具体来说，ORP 在 0 到 36 小时内控制在-200mV，在 36 到 156 小时内控制在-300mV，在 156 小时后控制在-400mV。PA 产量从 21.38 增加到 27.31g/L。研究了 ORP 调节对关键细胞内代谢物的影响，表明 ORP 既能调节 NADH/NAD(+) 比，又能调节电子传递过程中涉及的一些酶的活性，并重新分配代谢通量。我们将 ORP 控制策略与分批补料培养方法相结合，将 PA 产量提高到 39.53g/L。这种新的 ORP 控制策略可能有助于优化其他厌氧过程。
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