State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 5 Xinmofan Road, New Model Road No. 5, Nanjing, People's Republic of China.
Bioprocess Biosyst Eng. 2010 Nov;33(9):1077-85. doi: 10.1007/s00449-010-0433-7. Epub 2010 Jun 30.
Propionic acid was produced in a multi-point fibrous-bed (MFB) bioreactor by Propionibacterium freudenreichii CCTCC M207015. The MFB bioreactor, comprising spiral cotton fiber packed in a modified 7.5-l bioreactor, was effective for cell-immobilized propionic acid production compared with conventional free cell fermentation. Batch fermentations at various glucose concentrations were investigated in the MFB bioreactor. Based on analysis of the time course of production, a fed-batch strategy was applied for propionic acid production. The maximum propionic acid concentration was 67.05 g l(-1) after 496 h of fermentation, and the proportion of propionic acid to total organic acids was approximately 78.28% (w/w). The MFB bioreactor exhibited excellent production stability during batch fermentation and the propionic acid productivity remained high after 78 days of fermentation.
丙酸由弗氏丙酸杆菌 CCTCC M207015 在多点纤维床（MFB）生物反应器中产生。与传统的游离细胞发酵相比，包含螺旋棉纤维填充的改良 7.5 升生物反应器的 MFB 生物反应器对于细胞固定化丙酸生产非常有效。在 MFB 生物反应器中研究了不同葡萄糖浓度下的分批发酵。基于对生产时间过程的分析，采用补料分批策略进行丙酸生产。发酵 496 小时后，丙酸的最大浓度为 67.05 g l(-1)，丙酸与总有机酸的比例约为 78.28%（w/w）。在分批发酵过程中，MFB 生物反应器表现出出色的生产稳定性，并且在发酵 78 天后丙酸生产率仍然很高。
