Marelli Belkis E, Diaz Pablo U, Salvetti Natalia R, Rey Florencia, Ortega Hugo H
Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Consejo Nacional de Investigaciones Científicas y Tecnológicas, (CONICET), Argentina.
Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Consejo Nacional de Investigaciones Científicas y Tecnológicas, (CONICET), Argentina.
Reprod Biol. 2014 Dec;14(4):276-81. doi: 10.1016/j.repbio.2014.08.002. Epub 2014 Aug 30.
Follicular growth and steroidogenesis are dependent on gonadotropin binding to their receptors in granulosa and theca cells of ovarian follicles. The aim of the present study was to evaluate the expression patterns of follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHCGR) in ovarian follicular structures from cows with cystic ovarian disease (COD) as compared with those of regularly cycling cows. Relative real-time RT-PCR analysis showed that the expression of FSHR mRNA in granulosa cells was highest in small antral follicles, then decreased significantly as follicles increased in size, and was lowest in cysts. FSHR mRNA was not detected in the theca cells of any follicular category, including cysts. LHCGR mRNA expression in granulosa cells was significantly higher in large antral follicles than in cysts, and not detected in granulosa cells of small and medium antral follicles. In theca cells, the expression level of LHCGR mRNA in medium antral follicles was higher than in small and large antral follicles, whereas that in follicular cysts it was similar to those in small and medium antral follicles, but higher than that in large antral follicles. Our findings provide evidence that there is an altered gonadotropin receptor expression in bovine cystic follicles, and suggest that in conditions characterized by altered ovulation, such as COD, changes in the signaling system of gonadotropins may play a fundamental role in their pathogenesis.
卵泡生长和类固醇生成依赖于促性腺激素与其在卵巢卵泡颗粒细胞和膜细胞中的受体结合。本研究的目的是评估与正常发情周期奶牛相比，患有卵巢囊肿疾病（COD）的奶牛卵巢卵泡结构中促卵泡激素受体（FSHR）和促黄体生成素受体（LHCGR）的表达模式。相对实时RT-PCR分析表明，颗粒细胞中FSHR mRNA的表达在小腔卵泡中最高，然后随着卵泡大小的增加而显著降低，在囊肿中最低。在任何卵泡类型（包括囊肿）的膜细胞中均未检测到FSHR mRNA。颗粒细胞中LHCGR mRNA的表达在大腔卵泡中显著高于囊肿，在中小腔卵泡的颗粒细胞中未检测到。在膜细胞中，中腔卵泡中LHCGR mRNA的表达水平高于小腔和大腔卵泡，而在卵泡囊肿中，其表达水平与中小腔卵泡相似，但高于大腔卵泡。我们的研究结果提供了证据，表明牛囊肿卵泡中促性腺激素受体表达发生了改变，并表明在以排卵改变为特征的情况下，如COD，促性腺激素信号系统的变化可能在其发病机制中起重要作用。
