Türkmen Deniz, Denizli Adil
Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.
Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.
Colloids Surf B Biointerfaces. 2014 Nov 1;123:859-65. doi: 10.1016/j.colsurfb.2014.10.034. Epub 2014 Oct 25.
The purpose of this study is to synthesize megaporous cryogel loaded with hydrophobic affinity beads which can be utilized for the purification of lysozyme from chicken egg-white. N-methacryloyl-(L)-tryptophan methylester (MATrp) was used as the hydrophobic ligand. In the first step, poly(glycidyl methacrylate-N-methacryloyl-(L)-tryptophan methyl-ester) [PGMATrp] beads (2.2 μm in diameter) were produced by dispersion polymerization. In the second step, the poly(2-hydroxyethyl methacrylate) (PHEMA) cryogel loaded with PGMATrp beads [PHEMA/PGMATrp composite cryogel] was polymerized initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) at -12°C. Lysozyme adsorption capacity of the PHEMA/PGMATrp composite cryogel (332.7 mg/g polymer) was improved significantly due to the loading of PGMATrp beads into the cryogel structure. The hydrophobic MATrp comonomer played a vital role in this binding mechanism. The PHEMA/PGMATrp composite cryogel could be used many times without decreasing the lysozyme adsorption amount significantly. The main advantage of the PHEMA/PGMATrp composite cryogel is the high adsorption capacity.
本研究的目的是合成负载疏水亲和珠的大孔冷冻凝胶，其可用于从鸡蛋白中纯化溶菌酶。N-甲基丙烯酰基-(L)-色氨酸甲酯（MATrp）用作疏水配体。第一步，通过分散聚合制备直径为2.2μm的聚（甲基丙烯酸缩水甘油酯-N-甲基丙烯酰基-(L)-色氨酸甲酯）[PGMATrp]珠。第二步，在-12°C下，由N，N，N'，N'-四亚甲基二胺（TEMED）和过硫酸铵（APS）引发聚合负载PGMATrp珠的聚（甲基丙烯酸2-羟乙酯）（PHEMA）冷冻凝胶[PHEMA/PGMATrp复合冷冻凝胶]。由于PGMATrp珠负载到冷冻凝胶结构中，PHEMA/PGMATrp复合冷冻凝胶的溶菌酶吸附容量（332.7mg/g聚合物）显著提高。疏水的MATrp共聚单体在这种结合机制中起关键作用。PHEMA/PGMATrp复合冷冻凝胶可以多次使用而不会显著降低溶菌酶吸附量。PHEMA/PGMATrp复合冷冻凝胶的主要优点是吸附容量高。
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