没食子酸对羟基磷灰石晶体形态和生长的影响。
Effects of gallic acid on the morphology and growth of hydroxyapatite crystals.
作者信息
Tang Bei, Yuan He, Cheng Lei, Zhou Xuedong, Huang Xuelian, Li Jiyao
机构信息
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Department of Endodontics and Operative Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Department of Endodontics and Operative Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
出版信息
Arch Oral Biol. 2015 Jan;60(1):167-73. doi: 10.1016/j.archoralbio.2014.09.011. Epub 2014 Oct 5.
OBJECTIVE
This study was designed to explore the effect of gallic acid (GA, one of the ingredients of chemical compounds from galla chinensis) on the morphology and growth of hydroxyapatite crystals.
METHODS
The crystals was produced by mixing CaCl2 and KH2PO4 with or without GA (4g/L) at room temperature for 3, 12, 24h and 3, 7, 14 days. Subsequently, the micro-structure, morphology and composition of the crystals were investigated via SEM, XRD, ATR-FTIR and fluorescence microscopy.
RESULTS
The mineral phase was hydroxyapatite in both groups after 14 days, but their processes and the morphology were completely different. The crystals from groups utilizing GA for 14 days were urchin-like, while loose needle-like crystals were observed in groups without GA. XRD results indicated that GA might limit the growth of the crystals, mainly on the 002 direction. The results of ATR-FTIR and fluorescence microscopy revealed that the unique structures might caused by the participation of GA during crystals formation.
CONCLUSION
GA might affect and participate into the formation of the hydroxyapatite, and regulate the morphology and structure of the crystals, to enhance the remineralization process.
目的
本研究旨在探讨没食子酸（GA，五倍子化学成分之一）对羟基磷灰石晶体形态及生长的影响。
方法
通过在室温下将氯化钙和磷酸二氢钾与或不与GA（4g/L）混合3、12、24小时以及3、7、14天来制备晶体。随后，通过扫描电子显微镜（SEM）、X射线衍射（XRD）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）和荧光显微镜研究晶体的微观结构、形态和组成。
结果
14天后两组的矿物相均为羟基磷灰石，但它们的形成过程和形态完全不同。使用GA 14天的组中的晶体呈海胆状，而未使用GA的组中观察到松散的针状晶体。XRD结果表明，GA可能主要在002方向上限制晶体的生长。ATR-FTIR和荧光显微镜的结果表明，独特的结构可能是由于GA在晶体形成过程中的参与所致。
结论
GA可能影响并参与羟基磷灰石的形成，并调节晶体的形态和结构，以增强再矿化过程。
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