Oosterik Leon H, Tuntufye Huruma N, Butaye Patrick, Goddeeris Bruno M
Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium; Department of General Bacteriology, CODA-CERVA, Groeselenberg 99, 1180 Brussels, Belgium.
Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium; Department of Veterinary Microbiology and Parasitology, Sokoine University of Agriculture, P.O. Box 3019, Chuo Kikuu, 67125 Morogoro, Tanzania.
Vet J. 2014 Dec;202(3):561-5. doi: 10.1016/j.tvjl.2014.10.001. Epub 2014 Oct 7.
Avian pathogenic Escherichia coli (APEC) are responsible for significant economic losses in the poultry industry and are difficult to eradicate. Biofilm formation by APEC has the potential to reduce the efficacy of cleaning and disinfection. In this study, biofilm formation on materials used in poultry facilities by APEC strains from laying hens was determined. APEC strains were analysed for an association between biofilm forming capacity and O serogroup. The abilities of two routinely used disinfectants, hydrogen peroxide (H2O2) and a quaternary ammonium compound (QAC), to kill adherent cells of two strong APEC biofilm producers (05/503 and 04/40) and a non-biofilm producer (05/293) on polystyrene (PS) and polyvinylchloride (PVC) surfaces were tested. Most APEC strains were moderate (PS) or strong biofilm producers (polypropylene, PP, and PVC). Strains in serogroup O2 more often belonged to the moderate (PS) or strong (PP and PVC) biofilm producers than to other groups, while most O78 strains were weak biofilm producers. O78 strains were stronger biofilm producers on stainless steel than on PP and PVC, while O2 strains were stronger biofilm producers on PP and PVC. A concentration of 1% H2O2 killed all adherent bacteria of strains 05/503 and 04/40 on PP and PVC, while 0.5% H2O2 killed all adherent bacteria of strain 05/293. QAC at a concentration of 0.01% killed all adherent cells of strains 05/503, 04/40 and 05/293 under equal conditions. In conclusion, biofilm formation by APEC was affected by serogroup and surface material, and inactivation of APEC was dependent on the disinfectant and surface material.
禽致病性大肠杆菌（APEC）给家禽业造成了巨大经济损失，且难以根除。APEC形成生物膜有可能降低清洁和消毒的效果。在本研究中，测定了来自蛋鸡的APEC菌株在家禽设施所用材料上形成生物膜的情况。分析了APEC菌株生物膜形成能力与O血清群之间的关联。测试了两种常用消毒剂过氧化氢（H2O2）和季铵化合物（QAC）对聚苯乙烯（PS）和聚氯乙烯（PVC）表面上两种强APEC生物膜形成菌（05/503和04/40）以及一种非生物膜形成菌（05/293）的黏附细胞的杀灭能力。大多数APEC菌株是中度（PS）或强生物膜形成菌（聚丙烯，PP，和PVC）。O2血清群中的菌株比其他组更常属于中度（PS）或强（PP和PVC）生物膜形成菌，而大多数O78菌株是弱生物膜形成菌。O78菌株在不锈钢上比在PP和PVC上是更强的生物膜形成菌，而O2菌株在PP和PVC上是更强的生物膜形成菌。1%的H2O2浓度杀死了PP和PVC上05/503和04/40菌株的所有黏附细菌，而0.5%的H2O2杀死了05/293菌株的所有黏附细菌。0.01%浓度的QAC在相同条件下杀死了05/503、04/40和05/293菌株的所有黏附细胞。总之，APEC形成生物膜受血清群和表面材料影响，APEC的灭活取决于消毒剂和表面材料。
