Rodrigues Suelle V, Laviniki Vanessa, Borges Karen A, Furian Thales Q, Moraes Hamilton L S, Nascimento Vladimir P, Salle Carlos T P
Faculdade de Veterinária, Centro de Diagnóstico e Pesquisa em Patologia Aviária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090 - Bairro Agronomia, Porto Alegre, RS, CEP 91540-000, Brazil.
Faculdade de Veterinária, Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090 - Bairro Agronomia, Porto Alegre, RS, CEP 91540-000, Brazil.
Curr Microbiol. 2019 Feb;76(2):194-199. doi: 10.1007/s00284-018-1608-8. Epub 2018 Dec 4.
Avian pathogenic Escherichia coli (APEC) is one of the pathogens that most concerns the poultry industry worldwide due to the economic losses it can cause. APEC persistence and survival, both in the environment and in the host, may be a consequence of biofilm-producing capabilities. The aim of this study was to evaluate APEC strains' biofilm production and its relationship to in vivo pathogenicity. Two hundred thirty-eight APEC isolates from three different origins (broiler bedding material, cellulite lesions, and respiratory diseases) were selected. The in vivo pathogenicity index (PI) was determined. Biofilm formation was evaluated using a microplate assay with analysis of colony morphology in Congo Red agar in order to detect the phenotypic expression of curli fimbriae and cellulose. Regarding biofilm production, it was observed that 55.8% of the strains produced biofilms. In the morphological test, 88.2% of the isolates expressed one or both components at one of the temperatures at least, and 11.8% of the isolates did not express curli or cellulose. Cellulose production was significantly higher at 25 °C. On the other hand, curli production was significantly higher at 37 °C. The study data indicate that there is no association between biofilm production and in vivo pathogenicity.
禽致病性大肠杆菌（APEC）是全球家禽业最为关注的病原体之一，因其会造成经济损失。APEC在环境和宿主体内的持续存在和存活可能是其具有生物膜形成能力的结果。本研究的目的是评估APEC菌株的生物膜形成及其与体内致病性的关系。从三个不同来源（肉鸡垫料、脂肪团病变和呼吸道疾病）选取了238株APEC分离株。测定了体内致病性指数（PI）。使用微孔板测定法并通过分析刚果红琼脂中的菌落形态来评估生物膜形成，以检测卷曲菌毛和纤维素的表型表达。关于生物膜形成，观察到55.8%的菌株产生生物膜。在形态学试验中，88.2%的分离株至少在一个温度下表达一种或两种成分，11.8%的分离株不表达卷曲菌毛或纤维素。纤维素产量在25℃时显著更高。另一方面，卷曲菌毛产量在37℃时显著更高。研究数据表明生物膜形成与体内致病性之间没有关联。
