Sardari Roya R R, Dishisha Tarek, Pyo Sang-Hyun, Hatti-Kaul Rajni
J Biotechnol. 2014 Dec 20;192 Pt A:223-30. doi: 10.1016/j.jbiotec.2014.10.013.
3-Hydroxypropionaldehyde (3HPA), a potential C3-platform chemical for a biobased industry, is produced from glycerol using Lactobacillus reuteri through its glycerol dehydratase activity. However, the process is characterized by low yield and productivity due to toxic effects of 3HPA on the biocatalyst activity. In this study, a semicarbazide-functionalized resin was prepared, evaluated for adsorption and in situ recovery of 3HPA during biotransformation of glycerol. Adsorption of 3HPA onto the resin was characterized as “S-curve model”, increasing with increasing initial 3HPA concentration, and reached a maximum of 9.48 mmol/g(resin) at 71.54 mM 3HPA used. Desorption of 3HPA was evaluated using water and different acids, and was enhanced by acetic acid with organic modifiers. Repeated adsorption–desorption of 3HPA in batch resulted in elution of 13–66.5% of the bound 3HPA during at least three sequential cycles using water and acetic acid, respectively as eluants. Using the resin for in situ product removal led to more than 2 times higher productivity of 3HPA.
3-羟基丙醛（3HPA）是一种用于生物基产业的潜在C3平台化学品，通过罗伊氏乳杆菌的甘油脱水酶活性由甘油生产。然而，由于3HPA对生物催化剂活性的毒性作用，该过程的特点是产率和生产率较低。在本研究中，制备了一种氨基脲功能化树脂，并对其在甘油生物转化过程中吸附和原位回收3HPA的性能进行了评估。3HPA在树脂上的吸附表现为“S曲线模型”，随初始3HPA浓度的增加而增加，在使用71.54 mM 3HPA时达到最大值9.48 mmol/g（树脂）。使用水和不同的酸对3HPA的解吸进行了评估，乙酸与有机改性剂可增强解吸效果。在分批操作中对3HPA进行重复吸附-解吸，分别使用水和乙酸作为洗脱剂，在至少三个连续循环中可洗脱13%-66.5%的结合3HPA。使用该树脂进行原位产物去除可使3HPA的生产率提高2倍以上。
