Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden; Chemistry of Natural and Microbial Products Department, National Research Centre, Al-Bohoos St., 12622 Cairo, Egypt.
Bioresour Technol. 2015 Mar;180:172-6. doi: 10.1016/j.biortech.2014.12.099. Epub 2015 Jan 6.
Poly(3-hydroxypropionate), P(3HP), is a polymer combining good biodegradability with favorable material properties. In the present study, a production system for P(3HP) was designed, comprising conversion of glycerol to 3-hydroxypropionaldehyde (3HPA) as equilibrium mixture with 3HPA-hydrate and -dimer in aqueous system (reuterin) using resting cells of native Lactobacillus reuteri in a first stage followed by transformation of the 3HPA to P(3HP) using recombinant Escherichia coli strain co-expressing highly active coenzyme A-acylating propionaldehyde dehydrogenase (PduP) from L. reuteri and polyhydroxyalkanoate synthase (PhaCcs) from Chromobacterium sp. P(3HP) content of up to 40% (w/w) cell dry weight was reached, and the yield with respect to the reuterin consumed by the cells was 78%. Short biotransformation period (4.5h), lack of additives or expensive cofactors, and use of a cheap medium for cultivation of the recombinant strain, provides a new efficient and potentially economical system for P(3HP) production.
聚 3-羟基丙酸（P(3HP)）是一种兼具良好生物降解性和优良材料性能的聚合物。本研究设计了一种 P(3HP)生产系统，包括使用天然乳酸乳杆菌的静止细胞将甘油转化为 3-羟基丙醛（3HPA），3HPA 与水合和二聚体在水溶液中形成平衡混合物（雷替曲塞），然后使用重组大肠杆菌菌株将 3HPA 转化为 P(3HP)，该菌株共表达来自乳酸乳杆菌的高活性辅酶 A 酰化丙醛脱氢酶（PduP）和来自 Chromobacterium sp. 的聚羟基烷酸合酶（PhaCcs）。细胞干重中 P(3HP)的含量最高可达 40%（w/w），并且相对于细胞消耗的雷替曲塞，产率为 78%。较短的生物转化周期（4.5 小时）、缺乏添加剂或昂贵的辅因子，以及使用廉价的培养基培养重组菌株，为 P(3HP)生产提供了一种新的高效、潜在经济的系统。
