Borchman D, Paterson C A, Delamere N A
Department of Ophthalmology, University of Louisville School of Medicine, Kentucky Lions Eye Research Institute, Kentucky 40202.
Invest Ophthalmol Vis Sci. 1989 Jul;30(7):1633-7.
Hydrogen peroxide inhibition of maximum Ca2+-ATPase and Na+,K+-ATPase activity was measured in a membrane-enriched preparation of rabbit lens cortical fibers and epithelium. At 5 X 10(-6) M hydrogen peroxide maximum Ca2+-ATPase activity was inhibited by 39%, while maximum Na+,K+-ATPase activity was stimulated. Ca2+-ATPase activity was almost completely inhibited at 5 X 10(-4) M hydrogen peroxide, in comparison to Na+,K+-ATPase activity, which was only inhibited by 28% at a concentration of hydrogen peroxide an order of magnitude larger. The addition of catalase to hydrogen peroxide-pretreated samples did not reverse the inhibition of Ca2+-ATPase by hydrogen peroxide.
在兔晶状体皮质纤维和上皮细胞的富含膜的制剂中，测定了过氧化氢对最大Ca2 + -ATP酶和Na +，K + -ATP酶活性的抑制作用。在5×10（-6）M过氧化氢浓度下，最大Ca2 + -ATP酶活性被抑制39％，而最大Na +，K + -ATP酶活性受到刺激。与Na +，K + -ATP酶活性相比，在5×10（-4）M过氧化氢浓度下Ca2 + -ATP酶活性几乎完全被抑制，在过氧化氢浓度高一个数量级时，Na +，K + -ATP酶活性仅被抑制28％。向过氧化氢预处理的样品中添加过氧化氢酶并不能逆转过氧化氢对Ca2 + -ATP酶的抑制作用。
