Liu Xueke, Shen Zhigang, Wang Peng, Liu Chang, Zhou Zhiqiang, Liu Donghui
Department of Applied Chemistry, China Agricultural University, No.2 Yuanmingyuan West Road, Beijing 100193, PR China.
Department of Applied Chemistry, China Agricultural University, No.2 Yuanmingyuan West Road, Beijing 100193, PR China.
J Chromatogr A. 2014 Dec 5;1371:58-64. doi: 10.1016/j.chroma.2014.10.068. Epub 2014 Oct 31.
A novel effervescence assisted on-site liquid phase microextraction has been developed for the determination of five triazine herbicides in water. The use of an effervescent tablet composed of citric acid, sodium bicarbonate and 1-undecanol (extraction solvent) was the core of the method. The triazine herbicides in water were extracted by 1-undecanol released from tablet under effervescence and determined by ultra-high pressure liquid chromatography tandem mass spectrometer. The experimental variables, including NaCl concentration, temperature, weight of effervescent tablet, volume of extraction solvent and pH value, were screened by a Plackett-Burman design and optimized by a Box-Behnken design. Under the optimized conditions, good linearity was obtained in the range of 0.05-10 μg L(-1) with correlation coefficients ranging from 0.9936 to 0.9988. The limits of quantification were between 7.6 and 26.4 ng L(-1), and the recoveries were in 71.4-93.2% with relative standard deviations of 2.5-10.9%. This method, which does not require centrifugation and any special apparatus, was successfully applied to determine triazine herbicides in real waters, promising to be a way to speed field sampling procedures for the organic pollutants monitoring in water.
已开发出一种新型泡腾辅助现场液相微萃取方法用于测定水中的五种三嗪类除草剂。使用由柠檬酸、碳酸氢钠和1-十一醇（萃取溶剂）组成的泡腾片是该方法的核心。水中的三嗪类除草剂通过泡腾作用从泡腾片中释放出的1-十一醇进行萃取，并通过超高压液相色谱串联质谱仪进行测定。通过Plackett-Burman设计筛选包括氯化钠浓度、温度、泡腾片重量、萃取溶剂体积和pH值在内的实验变量，并通过Box-Behnken设计进行优化。在优化条件下，在0.05 - 10 μg L(-1)范围内获得了良好的线性，相关系数在0.9936至0.9988之间。定量限在7.6至26.4 ng L(-1)之间，回收率在71.4 - 93.2%之间，相对标准偏差为2.5 - 10.9%。该方法无需离心和任何特殊仪器，已成功应用于实际水样中三嗪类除草剂的测定，有望成为加快水中有机污染物监测现场采样程序的一种方法。
