Bârzu O, Guerrieri F, Scarfò R, Capozza G, Papa S
Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
J Bioenerg Biomembr. 1989 Jun;21(3):403-14. doi: 10.1007/BF00762730.
The amphiphylic alkyl cation cetyltrimethylammonium inhibits the catalytic activity of soluble and membrane-bound F1 in a noncompetitive fashion. In sonic submitochondrial particles the Dixon plot showed a peculiar pattern with upward deviation at cetyltrimethylammonium concentration higher than 80 microM. In membrane-bound F1 the inhibition by cetyltrimethylammonium was potentiated by the F0 inhibitor ologomycin. Cetyltrimethylammonium also inhibited the oligomycin-sensitive proton conductivity in F1-containing particles but was without any effect in F1-depleted particles. Also this inhibitory effect was potentiated by oligomycin. These results indicate functional cooperative interactions between F0 and F1.
两亲性烷基阳离子十六烷基三甲基铵以非竞争性方式抑制可溶性和膜结合型F1的催化活性。在超声处理的亚线粒体颗粒中，狄克逊图呈现出一种特殊模式，在十六烷基三甲基铵浓度高于80微摩尔时出现向上偏差。在膜结合型F1中，F0抑制剂寡霉素可增强十六烷基三甲基铵的抑制作用。十六烷基三甲基铵还抑制含F1颗粒中的寡霉素敏感质子传导性，但对不含F1的颗粒没有任何影响。这种抑制作用也被寡霉素增强。这些结果表明F0和F1之间存在功能协同相互作用。
