Varga N, Benkő M, Sebők D, Dékány I
Department of Physical Chemistry and Materials Science, Faculty of Science and Informatics, University of Szeged, Aradi Vt. 1, Szeged H-6720, Hungary.
MTA-SZTE Supramolecular and Nanostructured Materials Research Group, University of Szeged, Dóm tér. 8, Szeged H-6720, Hungary.
Colloids Surf B Biointerfaces. 2014 Nov 1;123:616-22. doi: 10.1016/j.colsurfb.2014.10.005. Epub 2014 Oct 27.
Bovine serum albumin (BSA) based core-shell nanoparticles were developed as carrier systems for drug transportation. At pH=3, the oppositely charged polyelectrolytes: poly(sodium-4-styrene)sulphonate (PSS) and the chitosan (Chit) bind to the positively charged protein via electrostatic interactions. We applied ibuprofen (IBU) as model molecule which has low solubility. The changes in the BSA's secondary structure during the steps of the synthesis were inspected by FT-IR measurements. The size and the zeta potential were determined by dynamic light scattering (DLS). The changes in the structure and in the size were investigated by small angle X-ray scattering (SAXS) too, for each composite. The release of the ibuprofen was studied by vertical diffusion cell (Franz cell) at pH 7.4 at 25 and 37.5°C. The structure of the core-shell nanoparticles have significantly changed as the pH has risen from 3.0 to 7.4. Kinetic models were used to describe the release mechanism. The experimental results demonstrated that the BSA has an ordered structure at pH=3 which will become random coil by adding ibuprofen. The first shell restores the ordered structure of the protein. The controlled release was carried out; the IBU release decreased by 40% in the case of two-layered composites compared with the "naked" BSA.
基于牛血清白蛋白（BSA）的核壳纳米颗粒被开发为药物运输的载体系统。在pH = 3时，带相反电荷的聚电解质：聚（4-苯乙烯磺酸钠）（PSS）和壳聚糖（Chit）通过静电相互作用与带正电荷的蛋白质结合。我们使用布洛芬（IBU）作为低溶解度的模型分子。通过傅里叶变换红外光谱（FT-IR）测量检查合成步骤中BSA二级结构的变化。通过动态光散射（DLS）测定尺寸和zeta电位。还通过小角X射线散射（SAXS）研究了每种复合材料的结构和尺寸变化。在25和37.5°C下，在pH 7.4时通过垂直扩散池（Franz池）研究布洛芬的释放。随着pH从3.0升高到7.4，核壳纳米颗粒的结构发生了显著变化。使用动力学模型描述释放机制。实验结果表明，BSA在pH = 3时具有有序结构，加入布洛芬后将变为无规卷曲。第一层壳恢复了蛋白质的有序结构。进行了控释；与“裸”BSA相比，两层复合材料的IBU释放降低了40％。
