Pedersen Lasse Eggers, Jungersen Gregers, Sorensen Maria Rathmann, Ho Chak-Sum, Vadekær Dorte Fink
The National Veterinary Institute, Technical University of Denmark, 1870 Frederiksberg C, Denmark.
The National Veterinary Institute, Technical University of Denmark, 1870 Frederiksberg C, Denmark.
Vet Immunol Immunopathol. 2014 Dec 15;162(3-4):108-16. doi: 10.1016/j.vetimm.2014.10.007. Epub 2014 Oct 23.
The swine major histocompatibility complex (MHC) genomic region (SLA) is extremely polymorphic comprising high numbers of different alleles, many encoding a distinct MHC class I molecule, which binds and presents endogenous peptides to circulating T cells of the immune system. Upon recognition of such peptide-MHC complexes (pMHC) naïve T cells can become activated and respond to a given pathogen leading to its elimination and the generation of memory cells. Hence SLA plays a crucial role in maintaining overall adaptive immunologic resistance to pathogens. Knowing which SLA alleles that are commonly occurring can be of great importance in regard to future vaccine development and the establishment of immune protection in swine through broad coverage, highly specific, subunit based vaccination against viruses such as swine influenza, porcine reproductive and respiratory syndrome virus, vesicular stomatitis virus, foot-and-mouth-disease virus and others. Here we present the use of low- and high-resolution PCR-based typing methods to identify individual and commonly occurring SLA class I alleles in Danish swine. A total of 101 animals from seven different herds were tested, and by low resolution typing the top four most frequent SLA class I alleles were those of the allele groups SLA-304XX, SLA-108XX, SLA-202XX, and SLA-107XX, respectively. Customised high resolution primers were used to identify specific alleles within the above mentioned allele groups as well as within the SLA-205XX allele group. Our studies also suggest the most common haplotype in Danish pigs to be Lr-4.0 expressing the SLA-104XX, SLA-204XX, and SLA-304XX allele combination.
猪主要组织相容性复合体(MHC)基因组区域(SLA)具有极高的多态性,包含大量不同的等位基因,其中许多编码独特的MHC I类分子,该分子可结合内源性肽并将其呈递给免疫系统的循环T细胞。在识别此类肽-MHC复合物(pMHC)后,未成熟T细胞可被激活并对特定病原体作出反应,从而导致病原体被清除并产生记忆细胞。因此,SLA在维持对病原体的整体适应性免疫抵抗中起着至关重要的作用。了解哪些常见的SLA等位基因对于未来疫苗开发以及通过针对猪流感、猪繁殖与呼吸综合征病毒、水疱性口炎病毒、口蹄疫病毒等病毒进行广泛覆盖、高度特异性、基于亚单位的疫苗接种来建立猪的免疫保护具有重要意义。在此,我们展示了使用基于低分辨率和高分辨率PCR的分型方法来鉴定丹麦猪中个体和常见的SLA I类等位基因。对来自七个不同猪群的总共101只动物进行了检测,通过低分辨率分型,最常见的前四个SLA I类等位基因分别是等位基因组SLA-304XX、SLA-108XX、SLA-202XX和SLA-107XX的等位基因。使用定制的高分辨率引物来鉴定上述等位基因组以及SLA-205XX等位基因组内的特定等位基因。我们的研究还表明,丹麦猪中最常见的单倍型是表达SLA-104XX、SLA-204XX和SLA-304XX等位基因组合的Lr-4.0。