Kawada Toru, Akiyama Tsuyoshi, Shimizu Shuji, Sata Yusuke, Turner Michael J, Shirai Mikiyasu, Sugimachi Masaru
Auton Neurosci. 2014 Dec;186:62-8. doi: 10.1016/j.autneu.2014.10.016.
Arterial pressure (AP) elevates as a logarithmic function of exogenously administered dose of norepinephrine (NE). In contrast, AP is nearly linearly correlated with efferent sympathetic nerve activity (SNA) during acute baroreflex intervention. The present study aimed at quantifying the relationship between SNA and plasma NE concentration during acute baroreflex intervention. Carotid sinus regions were isolated from systemic circulation in five Wistar Kyoto rats, and carotid sinus pressure was changed among 60, 100, 120, 140, and 180 mm Hg every 2 min. Arterial blood (0.2 ml) was obtained at each pressure level for plasma NE measurement. Maximum AP and minimum AP were 153.34 ± 6.28 and 67.31 ± 4.92 mm Hg, respectively, in response to pressure perturbation. Plasma NE correlated linearly with SNA for individual animal data (slope: 0.957 ± 0.090 pg · ml(-1) · %(-1), intercept: 46.57 ± 7.22 pg/ml, r(2): ranged from 0.923 to 0.992) and also for group averaged data (NE = 0.956 × SNA + 47.97, r(2 )= 0.982). Blockade of neuronal NE uptake by intravenous desipramine (1 mg/kg) administration increased the slope (2.966 ± 0.686 pg · ml(-1) · %(-1), P < 0.05) and the intercept (168.73 ± 28.53 pg/ml, P < 0.01) of the plasma NE-SNA relationship. These results indicate that the relationship between SNA and plasma NE concentration was nearly linear within the normal physiological range of acute baroreflex control of AP. While plasma NE concentration can reflect changes in SNA, it may also overestimate the sympathetic outflow from the central nervous system when neuronal NE uptake is impaired systemically.
动脉血压（AP）随着外源性给予去甲肾上腺素（NE）剂量的对数函数而升高。相比之下，在急性压力反射干预期间，AP与传出交感神经活动（SNA）几乎呈线性相关。本研究旨在量化急性压力反射干预期间SNA与血浆NE浓度之间的关系。在五只Wistar Kyoto大鼠中，将颈动脉窦区域与体循环分离，每隔2分钟将颈动脉窦压力在60、100、120、140和180 mmHg之间改变。在每个压力水平采集动脉血（0.2 ml）用于血浆NE测量。响应压力扰动时，最大AP和最小AP分别为153.34±6.28和67.31±4.92 mmHg。血浆NE与个体动物数据的SNA呈线性相关（斜率：0.957±0.090 pg·ml⁻¹·%⁻¹，截距：46.57±7.22 pg/ml，r²：范围为0.923至0.992），并且与组平均数据也呈线性相关（NE = 0.956×SNA + 47.97，r² = 0.982）。静脉注射地昔帕明（1 mg/kg）阻断神经元对NE的摄取增加了血浆NE - SNA关系的斜率（2.966±0.686 pg·ml⁻¹·%⁻¹，P < 0.05）和截距（168.73±28.53 pg/ml，P < 0.01）。这些结果表明，在急性压力反射控制AP的正常生理范围内，SNA与血浆NE浓度之间的关系几乎是线性的。虽然血浆NE浓度可以反映SNA的变化，但当神经元对NE的摄取在全身受到损害时，它也可能高估中枢神经系统的交感神经输出。
