Brown S, Irwin D, Anastassiades T
Department of Medicine, Queen's University, Kingston, ON, Canada.
J Rheumatol. 1989 Feb;16(2):209-16.
A bovine articular cartilage longterm culture system was used to study proteoglycan synthesis by a pulse labeling technique. A brief preincubation of the cartilage slices with bacterial collagenase was found to be an effective method of depleting proteoglycan. The addition of a fraction from bovine bone that contained partially purified "matrigenin" activity to the cultures resulted in the stimulation of incorporation of radioactive precursors into cartilage chondroitin sulfate, suggesting increased proteoglycan synthesis. The stimulatory effect of matrigenin activity was observed earlier and was more sustained if the cartilage slices were preincubated with collagenase. The system appears to be useful for testing the hypothesis that bone derived factors stimulate the repair of damaged cartilage.
采用牛关节软骨长期培养系统，通过脉冲标记技术研究蛋白聚糖的合成。发现用细菌胶原酶对软骨切片进行短暂预孵育是一种有效去除蛋白聚糖的方法。向培养物中添加来自牛骨的含有部分纯化的“基质生成素”活性的组分，导致放射性前体掺入软骨硫酸软骨素增加，提示蛋白聚糖合成增加。如果软骨切片用胶原酶预孵育，基质生成素活性的刺激作用出现得更早且更持久。该系统似乎有助于检验骨源性因子刺激受损软骨修复这一假说。
