Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil; INCT in Energy & Environment, Department of Chemistry, Federal University of Paraná, Brazil.
Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil.
Bioresour Technol. 2015 Jan;175:195-202. doi: 10.1016/j.biortech.2014.10.087. Epub 2014 Oct 23.
Hydrolysis of phosphoric acid-impregnated steam-treated sugarcane bagasse was pre-optimized using a face-centered central composite design in which the process variables were the substrate total solids (TS, %), agitation intensity (AI, rpm) and enzyme loading (EL, gg(-1)). Pretreatment was carried out at 180°C for 10min using cane bagasse with 50wt% moisture content containing 9.5mg of H3PO4 per gram of dry biomass. Hydrolyses were performed for 96h at 50°C using Cellic CTec2® and water-washed steam-treated substrates. The highest amount of fermentable sugars was obtained with 20wt% TS, producing 76.8gL(-1) of glucose equivalents, which corresponded to a total glucan conversion of 69.2wt% and to a theoretical net increase of 39% in ethanol production from the same sugarcane tonnage without considering the use of leaves, tops and the additional yields from C5 sugars.
用中心复合设计对磷酸浸渍蒸汽处理蔗渣进行水解条件的预优化，过程变量为底物总固体（TS，%）、搅拌强度（AI，rpm）和酶负荷（EL，gg(-1)）。预处理在 50wt%水分含量的甘蔗渣上进行，用 9.5mg 的 H3PO4 浸渍每克干生物质，温度为 180°C，时间 10min。水解在 50°C 下进行 96h，使用 Cellic CTec2®和水洗蒸汽处理的底物。用 20wt% TS 获得了最高量的可发酵糖，产生了 76.8gL(-1)的葡萄糖当量，相当于总葡聚糖转化率为 69.2wt%，从相同的甘蔗吨数计算，理论上乙醇产量增加了 39%，不考虑使用叶子、顶部和 C5 糖的额外产量。
