Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, China.
Bioresour Technol. 2015 Jan;175:473-9. doi: 10.1016/j.biortech.2014.10.111. Epub 2014 Oct 29.
In this study, sweet sorghum stem was subjected to hydrothermal pretreatment (HTP) and alkaline post-treatment to enhance its saccharification ratio by reducing its recalcitrance. The results showed that the HTP (110-210°C, 0.5-2.0h) significantly degraded hemicelluloses, and the pretreatment at the temperature higher than 190°C led to the partial degradation of the cellulose. As compared to the sole HTP, the integrated process removed most of lignin and hemicelluloses, which incurred a higher cellulose saccharification ratio. Under an optimum condition evaluated (HTP at 170°C for 0.5h and subsequent 2% NaOH treatment), 77.5% saccharification ratio was achieved, which was 1.8, 2.0 and 5.5 times as compared to the only HTP pretreated substrates, alkaline treated substrates alone and the raw material without pretreatment, respectively. Clearly, the integrated process can be considered as a promising approach to achieve an efficient conversion of lignocellulose to fermentable glucose.
在这项研究中，对甜高粱茎进行了水热预处理（HTP）和碱性后处理，以通过降低其抗降解性来提高糖化率。结果表明，HTP（110-210°C，0.5-2.0h）可显著降解半纤维素，且预处理温度高于 190°C 会导致纤维素部分降解。与单独的 HTP 相比，综合工艺去除了大部分木质素和半纤维素，从而提高了纤维素的糖化率。在评估的最佳条件下（170°C 下 HTP 处理 0.5h 后用 2%NaOH 处理），糖化率达到 77.5%，与仅进行 HTP 预处理的底物、仅用碱性处理的底物和未经预处理的原料相比，分别提高了 1.8、2.0 和 5.5 倍。显然，该综合工艺可被视为将木质纤维素高效转化为可发酵葡萄糖的一种有前景的方法。
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