Chen Chen, Chen Zhixiong, Chen Jiajun, Huang Jiawei, Li Huiling, Sun Shaolong, Liu Xiangdong, Wu Aimin, Wang Bo
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China.
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architectures, South China Agricultural University, Guangzhou 510642, China.
Polymers (Basel). 2020 Feb 5;12(2):340. doi: 10.3390/polym12020340.
The improvement of the saccharification of rice straw is one of the strategies to reduce the sophisticated pretreatment that results in high cost and is unfriendly to the environment. We explored the cell wall features in tetraploid rice and highlighted the enhanced saccharification of tetraploid with large biomass. Results showed that lignin content and S/G ratio reduced to 17.09% and 0.37, respectively, in tetraploid straw by the determination of the pyGC-MS method. After the pretreatment, the cellulose crystallinity index decreased from 63.22% to 57.65% in tetraploid straw, which is lower than that of pretreated diploid straw. Surface topological analysis of SEM images indicated that tetraploid straw was more susceptible to the pretreatment. Tetraploid straw showed a strong advantage in the process of enzymatic hydrolysis. The enzyme efficiency reached the highest value of 77.60%, and the rate of enzyme reaction was improved to make the reaction saturated earlier than conventional rice. We concluded that the high saccharification has resulted from the alteration of lignin and cellulose in tetraploid rice. Our research provides an improved green feedstock for bioenergy, and the tetraploid rice straw shows the potential utilization value in bioethanol production.
提高稻草糖化率是减少复杂预处理的策略之一，复杂预处理成本高且对环境不友好。我们探究了四倍体水稻的细胞壁特征，并强调了具有大量生物量的四倍体水稻糖化率的提高。结果表明，通过热解气相色谱 - 质谱联用（pyGC - MS）法测定，四倍体稻草中的木质素含量和S/G比分别降至17.09%和0.37。预处理后，四倍体稻草的纤维素结晶度指数从63.22%降至57.65%，低于预处理后的二倍体稻草。扫描电子显微镜（SEM）图像的表面拓扑分析表明，四倍体稻草更容易受到预处理的影响。四倍体稻草在酶水解过程中表现出强大优势。酶效率达到最高值77.60%，酶反应速率提高，使反应比常规水稻更早达到饱和。我们得出结论，四倍体水稻中木质素和纤维素的改变导致了高糖化率。我们的研究为生物能源提供了改良的绿色原料，四倍体稻草在生物乙醇生产中显示出潜在的利用价值。
