College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China.
Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China.
Bioresour Technol. 2015 Jan;175:529-36. doi: 10.1016/j.biortech.2014.10.160. Epub 2014 Nov 6.
The effect of two pretreatments methods, aqueous ammonia (SAA) and dilute acid (DA), on the chemical compositions, cellulose crystallinity, morphologic change, and enzymatic hydrolysis of bamboo fractions (bamboo yellow, timber, green, and knot) was compared. Bamboo fractions with SAA pretreatment had better hydrolysability than those with DA pretreatment. High crystallinity index resulted in low hydrolysis yield in the conversion of SAA pretreated bamboo fractions, not DA pretreated fractions. The increase of cellulase loading had modestly positive effect in the hydrolysis of both SAA and DA pretreated bamboo fractions, while supplement of xylanase significantly increased the hydrolysis of the pretreated bamboo fractions, especially after SAA pretreatment. The results indicated that SAA pretreatment was more effective than DA pretreatment in conversion of bamboo fractions, and supplementation of xylanase was necessary in effective conversion of the SAA pretreated fractions into fermentable sugars.
比较了两种预处理方法，氨水（SAA）和稀酸（DA），对竹部分（竹黄、木材、绿色和结）的化学成分、纤维素结晶度、形态变化和酶水解的影响。SAA 预处理的竹部分比 DA 预处理的竹部分具有更好的水解性能。高结晶度指数导致 SAA 预处理的竹部分的水解产率较低，而 DA 预处理的竹部分则没有。增加纤维素酶的用量对 SAA 和 DA 预处理的竹部分的水解都有适度的积极影响，而木聚糖酶的补充则显著提高了预处理竹部分的水解效率，尤其是在 SAA 预处理之后。结果表明，SAA 预处理比 DA 预处理更有效地转化竹部分，并且在将 SAA 预处理的部分有效转化为可发酵糖时需要补充木聚糖酶。
