College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China.
Molecules. 2020 Apr 16;25(8):1843. doi: 10.3390/molecules25081843.
A two-step process was proposed to enhance enzymatic hydrolysis of based on a comparative study of acid/alkaline pretreatments. Ferric chloride pretreatment (FP) effectively removed hemicellulose and recovered soluble sugars, but the enzymatic hydrolysis was not efficient. Dilute alkaline pretreatment (ALP) resulted in much better delignification and stronger morphological changes of the sample, making it more accessible to enzymes. While ALP obtained the highest sugar yield during enzymatic hydrolysis, the soluble sugar recovery from the pretreatment stage was still limited. Furthermore, a two-step ferric chloride and dilute alkaline pretreatment (F-ALP) has been successfully developed by effectively recovering soluble sugars in the first FP step and further removing lignin of the FP sample in the second ALP step to improve its enzymatic hydrolysis. As a result, the two-step process yielded the highest total sugar recovery (418.8 mg/g raw stalk) through the whole process.
提出了两步法来增强基于酸/碱预处理比较研究的纤维素酶水解。三氯化铁预处理（FP）有效地去除了半纤维素并回收了可溶性糖，但酶解效率不高。稀碱预处理（ALP）导致木质素脱除效果更好，样品形态变化更强，使酶更容易进入。虽然 ALP 在酶解过程中获得了最高的糖产率，但预处理阶段的可溶性糖回收仍然有限。此外，通过在第一步 FP 中有效回收可溶性糖，并在第二步 ALP 中进一步去除 FP 样品中的木质素来提高酶解效率，成功开发了两步法铁和稀碱预处理（F-ALP）。结果，两步法通过整个过程获得了最高的总糖回收率（418.8mg/g 原料茎）。
