Brablcová Lenka, Buriánková Iva, Badurová Pavlína, Chaudhary Prem Prashant, Rulík Martin
Department of Ecology and Environmental Sciences, Laboratory of Aquatic Microbial Ecology, Faculty of Science, Palacky University in Olomouc, Šlechtitelů 11, CZ-783 71 Olomouc, Czech Republic; Department of Biology, Faculty of Education, Palacky University in Olomouc, Žižkovo náměstí 5, CZ-771 40 Olomouc, Czech Republic.
Department of Ecology and Environmental Sciences, Laboratory of Aquatic Microbial Ecology, Faculty of Science, Palacky University in Olomouc, Šlechtitelů 11, CZ-783 71 Olomouc, Czech Republic; Department of Biology, Faculty of Education, Palacky University in Olomouc, Žižkovo náměstí 5, CZ-771 40 Olomouc, Czech Republic.
Anaerobe. 2015 Apr;32:24-31. doi: 10.1016/j.anaerobe.2014.11.009. Epub 2014 Nov 21.
Abundance and diversity of methanogenic archaea were studied at five localities along a longitudinal profile of a Sitka stream (Czech Republic). Samples of hyporheic sediments were collected from two sediment depths (0-25 cm and 25-50 cm) by freeze-core method. Methanogen community was analyzed by fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE) and sequencing method. The proportion of methanogens to the DAPI-stained cells varied among all localities and depths with an average value 2.08 × 10(5) per g of dry sediment with the range from 0.37 to 4.96 × 10(5) cells per g of dry sediment. A total of 73 bands were detected at 19 different positions on the DGGE gel and the highest methanogen diversity was found at the downstream located sites. There was no relationship between methanogen diversity and sediment depth. Cluster analysis of DGGE image showed three main clusters consisting of localities that differed in the number and similarity of the DGGE bands. Sequencing analysis of representative DGGE bands revealed phylotypes affiliated with members belonging to the orders Methanosarcinales, Methanomicrobiales and Methanocellales. The knowledge about occurrence and diversity of methanogenic archaea in freshwater ecosystems are essential for methane dynamics in river sediments and can contribute to the understanding of global warming process.
沿着一条锡特卡溪流（捷克共和国）的纵向剖面，在五个地点研究了产甲烷古菌的丰度和多样性。通过冷冻取芯法从两个沉积物深度（0 - 25厘米和25 - 50厘米）采集潜流沉积物样本。通过荧光原位杂交（FISH）、变性梯度凝胶电泳（DGGE）和测序方法分析产甲烷菌群落。产甲烷菌在所有地点和深度中占DAPI染色细胞的比例各不相同，平均每克干沉积物中有2.08×10⁵个，范围为每克干沉积物0.37至4.96×10⁵个细胞。在DGGE凝胶上的19个不同位置共检测到73条带，且在下游位置发现了最高的产甲烷菌多样性。产甲烷菌多样性与沉积物深度之间没有关系。DGGE图像的聚类分析显示三个主要聚类，由DGGE条带数量和相似性不同的地点组成。对代表性DGGE条带的测序分析揭示了与甲烷八叠球菌目、甲烷微菌目和甲烷杆菌目的成员相关的系统发育型。了解淡水生态系统中产甲烷古菌的存在和多样性对于河流沉积物中的甲烷动态至关重要，并有助于理解全球变暖过程。
