Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen 518060, China.
Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, Ghent 9000, Belgium.
Biosens Bioelectron. 2015 Apr 15;66:124-8. doi: 10.1016/j.bios.2014.11.004. Epub 2014 Nov 7.
In this paper, a rapid and sensitive multiplex flow-through immunoaffinity chromatography test (FTIACT) was developed for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk. The developed FTIACT method combines the purification, preconcentration and immunochemical detection of multiple antibiotics on the sepharose gel test layers. The use of liposome-encapsulated quantum dots (LQDs) with the FTIACT method exhibited the best results, with limits of detection (LODs) of 1 and 0.5ng/mL for the sulfonamides (SAs) and quinolones (QNs), respectively, through qualitative analysis (visual detection by the naked eye). In order to achieve low detection limit, the color intensity of the images were converted into relative optical density values to enable a quantitative evaluation. Quantitative analysis of the samples enabled the detection of SAs (0.13ng/mL) and QNs (0.062ng/mL) in spiked milk samples. The FTIACT described in this work shows promise as a multiplex immunoassay for the qualitative and quantitative screening of multiple chemical residues in milk.
本文建立了一种快速、灵敏的多残留同时免疫亲和层析检测方法，用于现场筛选牛奶中的 14 种磺胺类和 13 种喹诺酮类药物残留。该方法结合了琼脂糖凝胶测试层上的多种抗生素的净化、预浓缩和免疫化学检测。采用脂质体包裹的量子点（LQDs）与 FTIACT 方法结合使用，结果最佳，磺胺类（SAs）和喹诺酮类（QNs）的检测限（LOD）分别为 1 和 0.5ng/mL，可通过定性分析（肉眼可见）进行检测。为了实现低检测限，将图像的颜色强度转换为相对光密度值，以便进行定量评估。对实际样品的定量分析可以检测出牛奶中添加的磺胺类（0.13ng/mL）和喹诺酮类（0.062ng/mL）残留。本文所描述的 FTIACT 有望成为一种用于牛奶中多种化学残留的定性和定量筛选的多重免疫分析方法。
