Department of Life Sciences, The Natural History Museum, Cromwell Road, London SW7 5BD, United Kingdom.
Department of Physical-Chemistry, University of Cadiz, Poligono Industrial Rio San Pedro s/n, 11,510 Puerto Real, Cadiz, Spain.
Sci Total Environ. 2015 Feb 15;506-507:109-17. doi: 10.1016/j.scitotenv.2014.11.013. Epub 2014 Nov 17.
The relationship between the subcellular distribution of accumulated toxic metals into five operational fractions (subsequently combined into presumed detoxified and non-detoxified components) and toxicity in the clam Scrobicularia plana was investigated under different laboratory exposures. Clams were exposed to metal contaminated media (water and diet) and analysed for the partitioning of accumulated As, Cu and Zn into subcellular fractions. In general, metallothionein-like proteins, metal-rich granules and cellular debris in different proportions acted as main storage sites of accumulated metals in the clam soft tissues for these three metals. No significant differences were noted in the accumulation rates of As, Cu and Zn of groups of individuals with or without apparent signs of toxicity after up to 30 days of exposure to naturally contaminated sediment mixtures. There was, however, an increased proportional accumulation of Cu in the non-detoxified fraction with increased Cu accumulation rate in the clams, suggesting that the Cu uptake rate from contaminated sediments exceeded the combined rates of elimination and detoxification of Cu, with the subsequent likelihood for toxic effects in the clams.
研究了在不同实验室暴露条件下，贻贝 Scrobicularia plana 中积累的有毒金属亚细胞分布与毒性之间的关系（随后组合为假定解毒和未解毒成分）。贻贝暴露于受金属污染的介质（水和饮食）中，并分析了积累的砷、铜和锌在亚细胞级分中的分布。一般来说，金属硫蛋白样蛋白、富含金属的颗粒和不同比例的细胞碎片是这三种金属在贻贝软组织中积累的主要储存部位。在长达 30 天的时间里，暴露于自然污染的沉积物混合物中，具有或没有明显毒性迹象的个体组的砷、铜和锌的积累率没有显著差异。然而，随着贻贝中铜积累率的增加，非解毒级分中铜的比例积累增加，这表明从污染沉积物中吸收的铜的速率超过了铜的消除和解毒的综合速率，随后贻贝可能会产生毒性效应。
