Eskew M L, Zarkower A, Scheuchenzuber W J, Burgess J R, Scholz R W, Hildenbrandt G, Reddy C C
Pennsylvania State University, Dept. of Veterinary Science, University Park 16802.
Prostaglandins. 1989 Jul;38(1):79-89. doi: 10.1016/0090-6980(89)90018-x.
Effects of vitamin E and/or selenium (Se) deficiency on the secretion of arachidonic acid metabolites by zymosan-stimulated pulmonary alveolar macrophages (AM) were examined using cells from male Long-Evans hooded rats fed torula-yeast based diets with or without the supplementation of vitamin E (150 IU/kg) or Se (0.5 mg/kg). Alveolar macrophages obtained by lavage were purified by adherence and cultured for 4 h in Hank's balanced salt solution containing bovine serum albumin (0.1%) and zymosan (300 micrograms/ml). The arachidonic acid metabolites present in the culture supernatant were measured by radioimmunoassay. Altered vitamin E and Se nutrition had no effect on the number of cells or cell types recovered from the pulmonary airways. Alveolar macrophages derived from animals fed on diets deficient in vitamin E or Se or both nutrients secreted higher levels of prostaglandin E2 and thromboxane B2. Levels of both 5-hydroxyeicosatetraenoic acid and leukotriene B4 were significantly increased only in the group fed the diet adequate in Se but deficient in vitamin E. Our data suggest that vitamin E and Se might play an important role to control the levels of several physiologically and pathologically important arachidonic acid metabolites.
利用雄性长-伊斯特曼有帽大鼠的细胞，研究了维生素E和/或硒（Se）缺乏对酵母聚糖刺激的肺泡巨噬细胞（AM）分泌花生四烯酸代谢产物的影响。这些大鼠喂食以圆酵母为基础的日粮，添加或不添加维生素E（150 IU/kg）或硒（0.5 mg/kg）。通过灌洗获得的肺泡巨噬细胞经贴壁纯化，并在含有牛血清白蛋白（0.1%）和酵母聚糖（300微克/毫升）的汉克平衡盐溶液中培养4小时。通过放射免疫测定法测量培养上清液中存在的花生四烯酸代谢产物。维生素E和硒营养状况的改变对从肺气道回收的细胞数量或细胞类型没有影响。来自喂食缺乏维生素E或硒或两种营养素日粮的动物的肺泡巨噬细胞分泌较高水平的前列腺素E2和血栓素B2。仅在喂食硒充足但维生素E缺乏日粮的组中，5-羟基二十碳四烯酸和白三烯B4的水平显著升高。我们的数据表明，维生素E和硒可能在控制几种生理和病理上重要的花生四烯酸代谢产物水平方面发挥重要作用。
