Ranohavimparany Anja, Bautin Nathalie, Fiamma Marie-Noëlle, Similowski Thomas, Straus Christian
Sorbonne Universités, UPMC Univ Paris 06, UMR_S 1158, Neurophysiologie Respiratoire Expérimentale et Clinique, F-75005 Paris, France; INSERM, UMR_S 1158, Neurophysiologie Respiratoire Expérimentale et Clinique, F-75005 Paris, France.
Sorbonne Universités, UPMC Univ Paris 06, UMR_S 1158, Neurophysiologie Respiratoire Expérimentale et Clinique, F-75005 Paris, France; INSERM, UMR_S 1158, Neurophysiologie Respiratoire Expérimentale et Clinique, F-75005 Paris, France; AP-HP, Groupe Hospitalier Pitié-Salpêtrière Charles Foix, Service d'Explorations Fonctionnelles de la Respiration, de l'Exercice et de la Dyspnée, F-75013 Paris, France.
Respir Physiol Neurobiol. 2016 Apr;224:27-36. doi: 10.1016/j.resp.2014.11.005. Epub 2014 Nov 13.
The neural respiratory output of isolated brainstems of post-metamorphic tadpoles displays two motor patterns, the buccal and the lung rhythms. Their global dynamics are complex and chaos-like. This study aimed at determining the source of this complexity. The neural respiratory output was recorded during exposure to increasing concentration of DAMGO or to reduced chloride concentration. Complexity was quantified with the noise limit (NL) and the largest Lyapunov exponent (LLE) values. DAMGO decreased lung frequency (p<0.0001), NL (p<0.0001) and LLE (p=0.0001) without changing buccal frequency (p=0.2392). Reduced concentration of chloride decreased buccal frequency (p=0.011) without changing lung frequency (p=0.2393) whereas NL (p=0.011) and LLE (p=0.027) increased significantly. When taking all the recordings into account, NL and LLE were correlated to lung frequency (r=0.661, p<0.0001 and r=0.3948, p=0.0012, respectively) but not to buccal frequency (r=0.1191, p=0.3487 and r=0.2083, p=0.0985, respectively). Therefore, the lung neural oscillator is both necessary and sufficient to the ventilatory complexity in the isolated brainstem of the post-metamorphic tadpole.
变态后蝌蚪离体脑干的神经呼吸输出表现出两种运动模式，即颊部节律和肺部节律。它们的整体动态是复杂且类似混沌的。本研究旨在确定这种复杂性的来源。在暴露于浓度不断增加的DAMGO或降低的氯离子浓度期间记录神经呼吸输出。用噪声极限（NL）和最大李雅普诺夫指数（LLE）值对复杂性进行量化。DAMGO降低了肺部频率（p<0.0001）、NL（p<0.0001）和LLE（p=0.0001），而未改变颊部频率（p=0.2392）。降低氯离子浓度降低了颊部频率（p=0.011），而未改变肺部频率（p=0.2393），而NL（p=0.011）和LLE（p=0.027）显著增加。当考虑所有记录时，NL和LLE与肺部频率相关（分别为r=0.661，p<0.0001和r=0.3948，p=0.0012），但与颊部频率无关（分别为r=0.1191，p=0.3487和r=0.2083，p=0.0985）。因此，肺部神经振荡器对于变态后蝌蚪离体脑干中的通气复杂性既是必要的也是充分的。
