11,12-白三烯A4：合成与代谢

11,12-leukotriene A4: synthesis and metabolism.

作者信息

Miki I, Kitamura S, Shimizu T, Seyama Y

机构信息

Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Adv Prostaglandin Thromboxane Leukot Res. 1989;19:98-101.

PMID:2546405

Abstract

11(S),12(S)-oxido-5Z,7E,9E,14Z-eicosatetraenoic acid (11,12-LTA4) was chemically synthesized from 12-hydroperoxy-eicosatetraenoic acid (12-HPETE). 11,12-LTA4 methyl ester was nonenzymically hydrolyzed to at least four products. These products were identified to be epimers of 5,12(S)-dihydroxy-eicosatetraenoic acid methyl ester (5,12(S)-diHETE-Me) and epimers of 11,12-diHETE-Me. In addition to the above nonenzymic products, 11,12-LTA4 was converted to 11,12-LTC4 by the rat liver glutathione S-transferase, and to an isomer of 11,12-diHETE by homogenates of the guinea pig adrenal gland and liver.

摘要

11(S),12(S)-环氧-5Z,7E,9E,14Z-二十碳四烯酸（11,12-LTA4）由12-氢过氧-二十碳四烯酸（12-HPETE）化学合成。11,12-LTA4甲酯经非酶水解生成至少四种产物。这些产物被鉴定为5,12(S)-二羟基-二十碳四烯酸甲酯（5,12(S)-diHETE-Me）的差向异构体和11,12-二羟基二十碳四烯酸甲酯（11,12-diHETE-Me）的差向异构体。除上述非酶产物外，11,12-LTA4被大鼠肝脏谷胱甘肽S-转移酶转化为11,12-LTC4，并被豚鼠肾上腺和肝脏匀浆转化为11,12-二羟基二十碳四烯酸的一种异构体。