Nakashima S, Tsunematsu Y, Nozawa Y
Nippon Ganka Gakkai Zasshi. 1989 Jan;93(1):149-54.
Ca2+ mobilization and phosphoinositide turnover were examined during phagocytosis of latex particles in cultured chick retinal pigment epithelial cells (RPE cells). Ca2+ influx into cells and Ca2+ efflux from the cells were enhanced by about 1.5-fold and 3-fold compared to control cells, respectively. A high content of Ca2+ in RPE cells has been reported. Therefore, the Ca2+ efflux observed here may be a reflection of Ca2+ release into cytosol from the intracellular storage site(s). In [3H] inositol-prelabeled RPE cells, addition of latex particles elicited decreases in phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol with the concomitant formation of inositol phosphates including inositol trisphosphate, indicating the hydrolysis of phosphoinositides by phospholipase C. These results suggest that phosphoinositide turnover may be closely coupled with Ca2+ mobilization during phagocytosis in RPE cells.
在培养的鸡视网膜色素上皮细胞（RPE细胞）吞噬乳胶颗粒的过程中，对钙离子动员和磷酸肌醇代谢进行了检测。与对照细胞相比，钙离子流入细胞和钙离子流出细胞分别增强了约1.5倍和3倍。据报道，RPE细胞中钙离子含量很高。因此，这里观察到的钙离子流出可能反映了钙离子从细胞内储存位点释放到细胞质中。在用[3H]肌醇预标记的RPE细胞中，添加乳胶颗粒会导致磷脂酰肌醇4,5-二磷酸和磷脂酰肌醇减少，同时形成包括肌醇三磷酸在内的肌醇磷酸，这表明磷脂酶C催化了磷酸肌醇的水解。这些结果表明，在RPE细胞吞噬过程中，磷酸肌醇代谢可能与钙离子动员密切相关。
