Bhatia Divya, Malik Deepak Kumar
J Environ Sci Eng. 2013 Apr;55(2):227-38.
Chlorpyrifos is being used globally as an agriculture based pesticide. Microbial degrada tion of chlorpyrifos pesticide is of particular interest because of high mammalian toxicity. Six bacterial cultures were isolated from chlorpyrifos contaminated soil by using enrich- ment technique. All the isolates showed good growth in basal salt medium containing chlorpyrifos and suggested their ability to utilize chlorpyrifos as sole carbon and energy source. All the six bacterial cultures were examined for various plant growth promoting properties, out of six only one bacteirial culture Pseudomonas aeruginosa DKC2 was found to be positive for various plant growth promoting activities (phosphate solubiliza- tion, protease activity, IAA production and antagonistic properties against plant patho- genic fungi). The resting cell study confirmed that the bacterial strain DKC2 degrade chlorpyrifos by 71 % within 2 days. Isolated bacterial culture can be used successfully for the removal of chlorpyrifos from contaminated soil along with plant growth promotion, but further research is required before commercial utilization of this isolate in removing chlorpyrifos from contaminated soil.
毒死蜱作为一种基于农业用途的杀虫剂在全球范围内被使用。由于其对哺乳动物具有高毒性，毒死蜱农药的微生物降解受到了特别关注。通过富集技术从受毒死蜱污染的土壤中分离出六种细菌培养物。所有分离物在含有毒死蜱的基础盐培养基中均表现出良好的生长，这表明它们有能力将毒死蜱作为唯一的碳源和能源加以利用。对所有六种细菌培养物进行了各种促进植物生长特性的检测，在这六种培养物中，只有一种细菌培养物铜绿假单胞菌DKC2被发现对各种促进植物生长的活动（磷溶解、蛋白酶活性、吲哚乙酸产生以及对植物致病真菌的拮抗特性）呈阳性。静息细胞研究证实，细菌菌株DKC2在两天内可将毒死蜱降解71%。分离出的细菌培养物可成功用于从受污染土壤中去除毒死蜱并促进植物生长，但在将该分离物用于商业去除受污染土壤中的毒死蜱之前，还需要进一步研究。
