Levakin I A, Losev E A, Zavirskiĭ Ia V, Galaktionov K V
Parazitologiia. 2013 Sep-Oct;47(5):353-60.
The study was carried out on Himasthla elongata cercariae shed by infected Littorina littorea snails. The infected periwinkles were collected from the settlement with the low prevalence of H. elongata. As shown earlier with the use of AFLP (Amplified Fragment Length Polymorphisms) method, rediae groups in all the infected periwinkles of this settlement arise from the infection of a mollusc with a single miracidium. Therefore, the cercariae shed by an infected mollusc have the same genotype or, in other words, represent a clone. The LT50 (the time during which 50% of cercariae perish in the experimental dish) were measured experimentally for cercariae Himasthla elongata belong to different clones. The investigated parameter demonstrated a high level of interclonal variability. Two groups of cercarial clones were identified: one of them was characterized by the high level of intraclonal variability in LT50 and the second, by the low one. It is assumed that the observed heterogeneity may be stipulated by different degrees of mitotic recombinations during formation of different cercarial clones.
该研究以感染的滨螺(Littorina littorea)排出的长茎希马斯吸虫尾蚴为研究对象。受感染的玉黍螺从长茎希马斯吸虫低流行率的定居点采集。如先前使用扩增片段长度多态性(AFLP)方法所示,该定居点所有受感染玉黍螺中的雷蚴群体均源自单个毛蚴对软体动物的感染。因此,受感染软体动物排出的尾蚴具有相同的基因型,或者换句话说,代表一个克隆。对属于不同克隆的长茎希马斯吸虫尾蚴进行实验测量其半数致死时间(LT50,即实验培养皿中50%尾蚴死亡的时间)。所研究的参数显示出高度的克隆间变异性。鉴定出两组尾蚴克隆:其中一组的特征是LT50的克隆内变异性高,另一组的特征是克隆内变异性低。据推测,观察到的异质性可能是由不同尾蚴克隆形成过程中有丝分裂重组的不同程度所规定的。