Solovyeva Anna, Nikolaev Kirill, Lebedev Egor, Potolytsina Edokiia, Galaktionov Nickolay, Levakin Ivan
Laboratory of Non-Coding DNA, Institute of Cytology of Russian Academy of Sciences, Laboratory of parasitic worms and protists, Zoological Institute of Russian Academy of Sciences, St Petersburg, Russia.
Laboratory of parasitic worms and protists, Zoological Institute of Russian Academy of Sciences, St.Petersburg, Russia.
J Helminthol. 2020 Feb 26;94:e129. doi: 10.1017/S0022149X20000103.
Digenean larvae of hermaphroditic generation - cercariae - are known to be polymorphic at genetic and behavioural levels. Cercariae arise as a result of parthenogenetic reproduction of intramolluscan stages, and represent a clone if a snail was infected with a single miracidium. Here we investigated cercarial clones of Himasthla elongata - namely, the infectivity of cercariae with normal (negative) and deviant (positive) photoreaction. In our study, most H. elongata clones showed intraclonal variance in their response to light. The proportion of photopositive cercariae ranged between 0.2% and 60% in different H. elongata clones. Photopositive larvae demonstrated significantly reduced rates of encystment in Mytilus edulis haemolymph in vitro and in young mussels. We discuss the possible mechanisms behind intraclonal variations, such as non-specific genomic rearrangements.
雌雄同体世代的复殖吸虫幼虫——尾蚴——在基因和行为水平上具有多态性，这是已知的。尾蚴是软体动物体内阶段孤雌生殖的结果，如果一只蜗牛被单个毛蚴感染，那么尾蚴就代表一个克隆体。在此，我们研究了长形希马斯吸虫的尾蚴克隆体——即具有正常（阴性）和异常（阳性）光反应的尾蚴的感染性。在我们的研究中，大多数长形希马斯吸虫克隆体在对光的反应上表现出克隆内差异。在不同的长形希马斯吸虫克隆体中，光阳性尾蚴的比例在0.2%至60%之间。光阳性幼虫在体外的紫贻贝血淋巴中和在幼贻贝中表现出明显降低的包囊形成率。我们讨论了克隆内变异背后的可能机制，如非特异性基因组重排。
