Schwachöfer J H, Crooijmans R P, Broers J L, Hoogenhout J, Jerusalem C R, Jerusalem R, Kal H B, Mungyer G
Department of Radiotherapy, University Hospital St. Radboud, Nijmegen, The Netherlands.
Anticancer Res. 1989 Mar-Apr;9(2):441-8.
Aggregates of human tumor cells are widely used in experimental studies on tumor responses to treatment. Only a limited number of human tumor cell lines are capable of forming spheroids. In this study cellular characteristics of 7 lung cancer and 4 bladder cancer cell lines are described with respect to their spheroid forming capacity. Comparisons were made with four reference lines known for their propensity to form growing aggregates. In the absence of vimentin expression no spherical aggregates were formed. Spherical aggregates were formed by one bladder and one lung cancer cell line, of which only the latter exhibited growth. Cellular factors influencing the ability of spheroids to increase in volume after spherical aggregation are not yet defined. Viability and clonogenicity of cells in aggregates are not the determinant of growth capacity. The growth rate of cell lines that exhibited growth is determined by tissue culture conditions and additives. Type of medium, percentage of foetal bovine serum and glucose concentration influenced the growth rate of spheroids. Since the response to radiation may be influenced by the growth rate of the tumors, manipulation of tissue culture medium composition offers the possibility of testing the influence of growth rate on the radiation response of one type of spheroids.
人类肿瘤细胞聚集体被广泛用于肿瘤治疗反应的实验研究。只有有限数量的人类肿瘤细胞系能够形成球体。在本研究中，描述了7种肺癌和4种膀胱癌细胞系关于其球体形成能力的细胞特征。与四种已知易于形成生长聚集体的参照细胞系进行了比较。在没有波形蛋白表达的情况下，未形成球形聚集体。一个膀胱癌细胞系和一个肺癌细胞系形成了球形聚集体，其中只有后者表现出生长。影响球体在球形聚集后体积增加能力的细胞因子尚未明确。聚集体中细胞的活力和克隆形成能力不是生长能力的决定因素。表现出生长的细胞系的生长速率由组织培养条件和添加剂决定。培养基类型、胎牛血清百分比和葡萄糖浓度影响球体的生长速率。由于对辐射的反应可能受肿瘤生长速率的影响，操纵组织培养基成分提供了测试生长速率对一种球体辐射反应影响的可能性。
