Li Min, Tong Xunliang, Lv Pu, Feng Baosheng, Yang Li, Wu Zheng, Cui Xinge, Bai Yu, Huang Yining, Liu Huwei
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Department of Neurology, Peking University First Hospital, Beijing 100034, China.
J Chromatogr A. 2014 Dec 12;1372C:110-119. doi: 10.1016/j.chroma.2014.10.094. Epub 2014 Nov 3.
A not-stop-flow online two-dimensional (2D) liquid chromatography (LC) method was developed for comprehensive lipid profiling by coupling normal- and reversed-phase LC with quadrupole time-of-flight mass spectrometry (QToF-MS), which was then applied to separate and identify the lipid species in plasma, making its merits in quality and quantity of the detection of lipids. Total 540 endogenous lipid species from 17 classes were determined in human plasma, and the differences in lipid metabolism products in human plasma between atherosclerosis patients and control subjects were explored in detail. The limit of detections (LODs) of 19 validation standards could all reach ng/mL magnitude, and the RSDs of peak area and retention time ranged 0.4-8.0% and 0.010-0.47%, respectively. In addition, a pair of isomers, galactosylceramides (GalC) and glucosylceramides (GluC), was successfully separated, showing that only the levels of GalC in atherosclerosis patients were significantly increasing, rather than GluC, compared with the controls (controls vs. patients: the ratio was 1.5-2.8-fold increasing). It would be helpful to the further research of the atherosclerosis.
通过将正相和反相液相色谱与四极杆飞行时间质谱（QToF-MS）联用，开发了一种用于全面脂质谱分析的非停流在线二维（2D）液相色谱（LC）方法，该方法随后应用于分离和鉴定血浆中的脂质种类，展现出其在脂质检测质量和数量方面的优势。在人血浆中测定了来自17类的总共540种内源性脂质种类，并详细探讨了动脉粥样硬化患者与对照受试者之间人血浆中脂质代谢产物的差异。19种验证标准品的检测限（LOD）均可达纳克/毫升级别，峰面积和保留时间的相对标准偏差（RSD）分别为0.4-8.0%和0.010-0.47%。此外，一对异构体，半乳糖神经酰胺（GalC）和葡萄糖神经酰胺（GluC）成功分离，结果表明与对照组相比，动脉粥样硬化患者中仅GalC水平显著升高，而非GluC（对照组与患者组：比值增加1.5-2.8倍）。这将有助于动脉粥样硬化的进一步研究。
