Zhang Zhen, Wang Li-Ping, Zhao Xian-Lan, Wang Fei, Huang Lan, Wang Meng, Chen Xin-Feng, Li Hong, Zhang Yi
Biotherapy Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
Biomed Pharmacother. 2014 Oct;68(8):1057-63. doi: 10.1016/j.biopha.2014.10.024. Epub 2014 Oct 31.
Cord blood (CB) is becoming an extensive source of cytokine-induced killer cells. It had been used in several clinical settings and proven to be efficacious and safe. Therefore, we investigated the possibility of combining CIK cells derived from cord blood (CB-CIK) and Pseudomonas aeruginosa injection (PA-MSHA) in order to enhance the cytotoxicity of CB-CIK cells against tumors. Compared with the CB-CIK cells, the PA-MSHA-treated CB-CIK cells demonstrated with increased proliferation rates, higher expression of activated cell surface marker CD28 and lower expression of inhibited cell surface markers PD-1 and CTLA-4. Furthermore, PA-MSHA-treated CB-CIK cells exhibited more effectively for secreting pro-inflammatory cytokine such as IFN-γ and expressing high levels of TLR2, TLR4 and TLR6. The expression of CD107a was higher in the CD3(+)CD56(+) subset of PA-MSHA-treated CB-CIK cells. Our results indicate that the PA-MSHA-treated CB-CIK cells exhibited a more potent in cytotoxic activity against tumor cells. Thus, PA-MSHA enhanced the antitumor ability of CB-CIK cells.
脐带血（CB）正成为细胞因子诱导的杀伤细胞的广泛来源。它已被用于多种临床环境，并被证明是有效且安全的。因此，我们研究了将源自脐带血的CIK细胞（CB-CIK）与铜绿假单胞菌注射液（PA-MSHA）联合使用的可能性，以增强CB-CIK细胞对肿瘤的细胞毒性。与CB-CIK细胞相比，经PA-MSHA处理的CB-CIK细胞显示出增殖率增加、活化细胞表面标志物CD28的表达更高以及抑制性细胞表面标志物PD-1和CTLA-4的表达更低。此外，经PA-MSHA处理的CB-CIK细胞更有效地分泌促炎细胞因子如IFN-γ，并高水平表达TLR2、TLR4和TLR6。在经PA-MSHA处理的CB-CIK细胞的CD3(+)CD56(+)亚群中，CD107a的表达更高。我们的结果表明，经PA-MSHA处理的CB-CIK细胞对肿瘤细胞表现出更强的细胞毒活性。因此，PA-MSHA增强了CB-CIK细胞的抗肿瘤能力。
