Wang Feng-Xue, Zhang Shu-Qin, Zhu Hong-Wei, Yang Yong, Sun Na, Tan Bin, Li Zhen-Guang, Cheng Shi-Peng, Fu Zhen F, Wen Yong-Jun
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China.
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences CAAS, Changchun 130112, Jilin, China; Department of Pathology, University of Georgia, Athens, GA 30602, USA.
Vet Microbiol. 2014 Dec 5;174(3-4):362-371. doi: 10.1016/j.vetmic.2014.10.023. Epub 2014 Oct 31.
The rabies virus (RV) vector LBNSE expressing foreign antigens have shown considerable promise as vaccines against viral and bacteria diseases, which is effective and safe. We produced a new RV-based vaccine vehicle expressing 1.824 kb hemagglutinin (H) gene of the canine distemper virus (CDV) by reverse genetics technology. The recombinant virus LBNSE-CDV-H retained growth properties similar to those of vector LBNSE both in BSR and mNA cell culture. The H gene of CDV was expressed and detected by immunostaining. To compare the immunogenicity of LBNSE-CDV-H, dogs were immunized with each of these recombinant viruses by intramuscular (i.m.). The dogs were bled at third weeks after the immunization for the measurement of virus neutralizing antibody (VNA) and then challenged with virulent virus (ZJ 7) at fourth weeks. The parent virus (LBNSE) without expression of any foreign molecules was included for comparison. Dogs inoculated with LBNSE-CDV-H showed no any signs of disease and exhibited seroconversion against both RV and CDV H protein. The LBNSE-CDV-H did not cause disease in dogs and conferred protection from challenge with a lethal wild type CDV strain, demonstrating its potential value for wildlife conservation efforts. Together, these studies suggest that recombinant RV expressing H protein from CDV stimulated high levels of adaptive immune responses (VNA), and protected all dogs challenge infection.
表达外源抗原的狂犬病病毒（RV）载体LBNSE作为针对病毒和细菌疾病的疫苗已显示出巨大的前景，它有效且安全。我们通过反向遗传学技术构建了一种新的基于RV的疫苗载体，该载体表达犬瘟热病毒（CDV）1.824 kb的血凝素（H）基因。重组病毒LBNSE-CDV-H在BSR和mNA细胞培养中保留了与载体LBNSE相似的生长特性。通过免疫染色检测到CDV的H基因得到了表达。为了比较LBNSE-CDV-H的免疫原性，用这些重组病毒分别对犬进行肌肉注射免疫。免疫后第三周采集犬的血液以检测病毒中和抗体（VNA），然后在第四周用强毒病毒（ZJ 7）进行攻毒。将未表达任何外源分子的亲本病毒（LBNSE）纳入作为对照。接种LBNSE-CDV-H的犬未出现任何疾病迹象，并且针对RV和CDV H蛋白均出现了血清转化。LBNSE-CDV-H在犬中未引发疾病，并且对致死性野生型CDV毒株的攻毒提供了保护，证明了其在野生动物保护方面的潜在价值。总之，这些研究表明，表达CDV H蛋白的重组RV刺激了高水平的适应性免疫反应（VNA），并保护所有犬免受攻毒感染。
