巴基斯坦萨拉基人群线粒体DNA控制区序列研究
Mitochondrial DNA control region sequences study in Saraiki population from Pakistan.
作者信息
Hayat Sikandar, Akhtar Tanveer, Siddiqi Muhammad Hassan, Rakha Allah, Haider Naeem, Tayyab Muhammad, Abbas Ghazanfar, Ali Azam, Bokhari Syed Yassir Abbas, Tariq Muhammad Akram, Khan Fazle Majid
机构信息
Department of Zoology, University of the Punjab, Lahore, Pakistan.
Department of Zoology, University of the Punjab, Lahore, Pakistan.
出版信息
Leg Med (Tokyo). 2015 Mar;17(2):140-4. doi: 10.1016/j.legalmed.2014.10.010. Epub 2014 Nov 8.
The analysis of mitochondrial DNA (mtDNA) control region was carried in 85 unrelated Sariki individuals living in the different provinces of Pakistan. DNA was extracted from blood preserved in EDTA vacutainers. Hypervariable regions (HV1, HV2 & HV3) were PCR amplified and sequenced. Sequencing results were aligned and compared with revised Cambridge reference sequence (rCRS). The sequencing results showed presence of total 63 different haplotypes, 58 of them are unique and 05 are common haplotypes shared by more than one individual. The most common haplotype observed was (W6) with a frequency 12.9% of population sample. The Saraiki population was detected with genetic diversity (0.9570) and power of discrimination (0.9458). This study will be beneficial for forensic casework.
对生活在巴基斯坦不同省份的85名无血缘关系的萨里基人进行了线粒体DNA（mtDNA）控制区分析。DNA从保存在乙二胺四乙酸（EDTA）真空采血管中的血液中提取。对高变区（HV1、HV2和HV3）进行聚合酶链反应（PCR）扩增和测序。将测序结果进行比对，并与修订后的剑桥参考序列（rCRS）进行比较。测序结果显示共存在63种不同的单倍型，其中58种是独特的，05种是多个个体共享的常见单倍型。观察到的最常见单倍型是（W6），在群体样本中的频率为12.9%。检测到萨里基人群的遗传多样性为（0.9570），鉴别能力为（0.9458）。本研究将有助于法医案件工作。
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