Stavnezer J, Lasky J L, Ponzio N M, Scheid M P, Thorbecke G J
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.
Eur J Immunol. 1989 Jun;19(6):1063-9. doi: 10.1002/eji.1830190616.
The immunoglobulin (Ig) heavy (H) and light (L) chain gene rearrangements of the high incidence SJL lymphomas (reticulum cell sarcoma, RCS) have been analyzed. Both primary and transplanted RCS show rearrangements of H and kappa L chains, demonstrating that these tumors are of B cell origin. These data are consistent with previous results indicating that these tumors are a mouse model for follicular lymphoma. A long-term transplanted line and the in vitro line derived from it, cRCS-X, have a single rearranged JH-C gamma 2a fragment and one rearranged C alpha gene fragment which does not hybridize with a probe for the JH gene segments. These cell lines also have two rearranged J kappa-C kappa fragments. Primary tumors and early passages are more heterogeneous with respect to Ig gene rearrangements, possibly because more than one B cell clone is present. Although no synthesis of IgG2a, or of any Ig, could be detected by the in vitro cRCS-X cells, these cells contain abundant poly(A)+ RNA that hybridize with gamma 2a and kappa probes as well as lesser amounts of alpha and epsilon RNA. None of these H chain RNA hybridized with probes for the JH gene segments. The epsilon and alpha RNA are the same size as transcripts of germ-line CH genes which have been identified in other systems. However, the gamma 2a RNA are smaller than previously described germ-line C gamma 2a RNA and appear to be transcribed from aberrantly rearranged JH-C gamma 2a genes.
对高发性SJL淋巴瘤（网状细胞肉瘤，RCS）的免疫球蛋白（Ig）重链（H）和轻链（L）基因重排进行了分析。原发性和移植性RCS均显示H链和κ轻链重排，表明这些肿瘤起源于B细胞。这些数据与先前的结果一致，表明这些肿瘤是滤泡性淋巴瘤的小鼠模型。一个长期移植系及其衍生的体外系cRCS-X，有一个单一的重排JH-Cγ2a片段和一个重排的Cα基因片段，该片段不与JH基因片段探针杂交。这些细胞系也有两个重排的Jκ-Cκ片段。原发性肿瘤和早期传代在Ig基因重排方面更具异质性，可能是因为存在多个B细胞克隆。尽管体外培养的cRCS-X细胞未检测到IgG2a或任何Ig的合成，但这些细胞含有丰富的多聚腺苷酸（poly(A)+）RNA，可与γ2a和κ探针杂交，以及少量的α和εRNA。这些重链RNA均未与JH基因片段探针杂交。ε和αRNA的大小与在其他系统中鉴定的种系CH基因转录本相同。然而，γ2a RNA比先前描述的种系Cγ2a RNA小，似乎是从异常重排的JH-Cγ2a基因转录而来。
