Park Byung Sun, Jo Hyun Woo, Jung Junyang
Department of Anatomy and Neurobiology, School of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
J Mol Histol. 2015 Feb;46(1):115-22. doi: 10.1007/s10735-014-9601-4. Epub 2014 Dec 3.
Aminoacyl-tRNA synthetases (AminoARSs) are essential enzymes involved in acylating tRNA with amino acids. In addition to the typical functions of AminoARSs, various non-canonical functions have been reported, such as involvement in cellular regulatory processes and signal transduction. Here, to explore the cellular changes in sensory neurons after nerve injury, we evaluated AARS mRNA expression in rat dorsal root ganglia (DRG) neurons using AminoARS-specific primers. Of 20 AminoARSs, we found that expression of lysyl-tRNA synthetase (KARS) and glutaminyl-tRNA synthetase (QARS) was decreased in the DRG injured side. We observed decreased KARS and QARS expression in DRG neuronal cell bodies, but not in satellite cells. Therefore, we suggest the possibility that KARS and QARS may act as signaling molecules to transfer abnormal sensory signals to the spinal dorsal horn after peripheral nerve damage. Therefore, KARS and QARS may represent powerful pharmaceutical targets via control of their non-canonical functions.
氨酰-tRNA合成酶（AminoARSs）是一类参与用氨基酸酰化tRNA的关键酶。除了AminoARSs的典型功能外，还报道了其多种非经典功能，如参与细胞调节过程和信号转导。在此，为了探究神经损伤后感觉神经元的细胞变化，我们使用AminoARS特异性引物评估了大鼠背根神经节（DRG）神经元中AARS mRNA的表达。在20种AminoARSs中，我们发现损伤侧DRG中赖氨酰-tRNA合成酶（KARS）和谷氨酰胺-tRNA合成酶（QARS）的表达降低。我们观察到DRG神经元细胞体中KARS和QARS的表达降低，但卫星细胞中未降低。因此，我们推测KARS和QARS可能作为信号分子，在周围神经损伤后将异常感觉信号传递至脊髓背角。因此，通过控制其非经典功能，KARS和QARS可能成为有力的药物靶点。
