Torresani J, Ghysdael J, Bismuth J, Teboul M, Ghiringhelli O, Savary J
INSERM U 38, CNRS URA 178, Laboratoire de Biochimie Médicale, Faculté de Médecine, Marseille, France.
FEBS Lett. 1989 Jul 3;250(2):251-6. doi: 10.1016/0014-5793(89)80732-x.
Polyclonal antibodies raised in rabbits against bacterially produced peptides in the C-terminal region of v-erb A or human c-erb A alpha oncogenes recognize the nuclear triiodothyronine (T3) receptors in the T3-sensitive Ob 17 mouse preadipocyte cell line and not in mouse or rat liver. The results confirm the existence of different T3 receptors in different tissues. The results also suggest a heterogeneous receptor distribution within the preadipose cell line, with a predominance of c-erb A alpha-type species. Antibodies raised against domain 149 227, but not against domain 245-325, impair T3 binding, suggesting a role for this domain in ligand binding.
在兔子体内产生的针对v-erb A或人c-erb Aα癌基因C末端区域细菌产生的肽段的多克隆抗体，能识别T3敏感的Ob 17小鼠前脂肪细胞系中的核三碘甲状腺原氨酸（T3）受体，而不能识别小鼠或大鼠肝脏中的该受体。结果证实不同组织中存在不同的T3受体。结果还表明在前脂肪细胞系中受体分布不均一，以c-erb Aα型为主。针对149 - 227结构域而非245 - 325结构域产生的抗体损害T3结合，表明该结构域在配体结合中起作用。
