Ceinos Rosa M, Guillot Raúl, Kelsh Robert N, Cerdá-Reverter José M, Rotllant Josep
Aquatic Molecular Pathobiology Group, Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Cientificas (CSIC), Vigo, Spain.
Pigment Cell Melanoma Res. 2015 Mar;28(2):196-209. doi: 10.1111/pcmr.12335. Epub 2014 Dec 22.
Dorso-ventral pigment pattern differences are the most widespread pigmentary adaptations in vertebrates. In mammals, this pattern is controlled by regulating melanin chemistry in melanocytes using a protein, agouti-signalling peptide (ASIP). In fish, studies of pigment patterning have focused on stripe formation, identifying a core striping mechanism dependent upon interactions between different pigment cell types. In contrast, mechanisms driving the dorso-ventral countershading pattern have been overlooked. Here, we demonstrate that, in fact, zebrafish utilize two distinct adult pigment patterning mechanisms - an ancient dorso-ventral patterning mechanism, and a more recent striping mechanism based on cell-cell interactions; remarkably, the dorso-ventral patterning mechanism also utilizes ASIP. These two mechanisms function largely independently, with resultant patterns superimposed to give the full pattern.
背腹色素模式差异是脊椎动物中最广泛存在的色素适应性变化。在哺乳动物中，这种模式是通过一种名为刺鼠信号肽（ASIP）的蛋白质调节黑素细胞中的黑色素化学来控制的。在鱼类中，色素模式形成的研究主要集中在条纹形成上，确定了一种依赖于不同色素细胞类型之间相互作用的核心条纹形成机制。相比之下，驱动背腹反荫蔽模式的机制一直被忽视。在这里，我们证明，事实上，斑马鱼利用两种不同的成体色素模式形成机制——一种古老的背腹模式形成机制，以及一种基于细胞间相互作用的更新的条纹形成机制；值得注意的是，背腹模式形成机制也利用了ASIP。这两种机制在很大程度上独立发挥作用，最终的模式相互叠加形成完整的模式。
